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Analysis of Vero cell growth behavior on microcarrier by means of environmental scanning electron microscopy
Alternative TitleSci. China Ser. C-Life Sci.
Shao, MJ; Jiang, L; Cong, W; Ouyang, F
2002-04-01
Source PublicationSCIENCE IN CHINA SERIES C-LIFE SCIENCES
ISSN1006-9305
Volume45Issue:2Pages:149-158
AbstractBy using environmental scanning electron microscopy, the morphological changes of Vero cells attached to and grown on the microcarrier Cytodex-3 were observed, and their behavior of adhesion, spreading and proliferation was analyzed. The effect of exogenous fibronectin/laminin on adhesion and spreading of MCC/Vero cell was studied. The images of ESEM showed that expansion of cell growth was directed toward vacancy space. The growth curve and cell concentration change during the whole culture process were obtained from the statistical counting method based on ESEM images and the crystal violet method. The growth rate of Vero cells increases with increasing the concentration of cell inoculation, that is, the specific growth rate increases quickly with increasing the concentration of cell inoculation. When serum concentration in medium #199 ranged from 5% to 10%, experimental results indicated that serum concentration is one of the important factors influencing cell growth, particularly in the cell adhesion and spreading stage.; By using environmental scanning electron microscopy, the morphological changes of Vero cells attached to and grown on the microcarrier Cytodex-3 were observed, and their behavior of adhesion, spreading and proliferation was analyzed. The effect of exogenous fibronectin/laminin on adhesion and spreading of MCC/Vero cell was studied. The images of ESEM showed that expansion of cell growth was directed toward vacancy space. The growth curve and cell concentration change during the whole culture process were obtained from the statistical counting method based on ESEM images and the crystal violet method. The growth rate of Vero cells increases with increasing the concentration of cell inoculation, that is, the specific growth rate increases quickly with increasing the concentration of cell inoculation. When serum concentration in medium #199 ranged from 5% to 10%, experimental results indicated that serum concentration is one of the important factors influencing cell growth, particularly in the cell adhesion and spreading stage.
KeywordGrowth Of Vero Cell Microcarrier Cytodex-3 Environmental Electron Scanning Microscopy
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
URL查看原文
Indexed BySCI
Language英语
WOS Research AreaLife Sciences & Biomedicine - Other Topics
WOS SubjectBiology
WOS IDWOS:000175009100005
Citation statistics
Cited Times:3[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Version出版稿
Identifierhttp://ir.ipe.ac.cn/handle/122111/5640
Collection研究所(批量导入)
Affiliation1.Chinese Acad Sci, Inst Chem Met, Multiphase React Lab, Beijing 100080, Peoples R China
2.Chinese Acad Sci, State Key Lab Biochem Engn, Beijing 100080, Peoples R China
Recommended Citation
GB/T 7714
Shao, MJ,Jiang, L,Cong, W,et al. Analysis of Vero cell growth behavior on microcarrier by means of environmental scanning electron microscopy[J]. SCIENCE IN CHINA SERIES C-LIFE SCIENCES,2002,45(2):149-158.
APA Shao, MJ,Jiang, L,Cong, W,&Ouyang, F.(2002).Analysis of Vero cell growth behavior on microcarrier by means of environmental scanning electron microscopy.SCIENCE IN CHINA SERIES C-LIFE SCIENCES,45(2),149-158.
MLA Shao, MJ,et al."Analysis of Vero cell growth behavior on microcarrier by means of environmental scanning electron microscopy".SCIENCE IN CHINA SERIES C-LIFE SCIENCES 45.2(2002):149-158.
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