Knowledge Management System Of Institute of process engineering,CAS
| Cellular Affinity of Particle-Stabilized Emulsion to Boost Antigen Internalization | |
| Cao, Fengqiang1,2,3; Ming, Yali3,4; Gao, Weixiang3,4; Ge, Jia3; Ogino, Kenji1,2 | |
| 2022-09-01 | |
| Source Publication | JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
 |
| ISSN | 1940-087X |
| Issue | 187Pages:18 |
| Abstract | The cellular affinity of micro-/nanoparticles is the precondition for cellular recognition, cellular uptake, and activation, which are essential for drug delivery and immune response. The present study stemmed from the observation that the effects of charge, size, and shape of solid particles on cell affinity are usually considered, but we seldom realize the essential role of softness, dynamic restructuring phenomenon, and complex interface interaction in cellular affinity. Here, we developed poly-lactic-co-glycolic acid (PLGA) nanoparticle-stabilized Pickering emulsion (PNPE) that overcame the shortcomings of rigid forms and simulated the flexibility and fluidity of pathogens. A method was set up to test the affinity of PNPE to cell surfaces and elaborate on the subsequent internalization by immune cells. The affinity of PNPE to bio-mimetic extracellular vesicles (bEVs)-the replacement for bone marrow dendritic cells (BMDCs)-was determined using a quartz crystal microbalance with dissipation monitoring (QCM-D), which allowed real-time monitoring of cell-emulsion adhesion. Subsequently, the PNPE was used to deliver the antigen (ovalbumin, OVA) and the uptake of the antigens by BMDCs was observed using confocal laser scanning microscope (CLSM). Representative results showed that the PNPE immediately decreased frequency (Delta F) when it encountered the bEVs, indicating rapid adhesion and high affinity of the PNPE to the BMDCs. PNPE showed significantly stronger binding to the cell membrane than PLGA microparticles (PMPs) and AddaVax adjuvant (denoted as surfactant-stabilized nano-emulsion [SSE]). Furthermore, owing to the enhanced cellular affinity to the immunocytes through dynamic curvature changes and lateral diffusions, antigen uptake was subsequently boosted compared with PMPs and SSE. This protocol provides insights for designing novel formulations with high cell affinity and efficient antigen internalization, providing a platform for the development of efficient vaccines. |
| DOI | 10.3791/64406 |
| Language | 英语 |
| WOS Keyword | DENDRITIC CELLS ; IMMUNE-RESPONSE ; MEMBRANE-FUSION ; DRUG-DELIVERY ; BINDING ; VIRUS ; SIZE |
| Funding Project | National Key Research and Development Program of China[2021YFE020527] ; National Key Research and Development Program of China[2021YFC2302605] ; National Key Research and Development Program of China[2021YFC2300142] ; Basic Frontier Scientific Research Program of Chinese Academy of Sciences[ZDBS-LY-SLH040] ; Foundation for Innovative Research Groups of the National Natural Science Foundation of China[21821005] |
| WOS Research Area | Science & Technology - Other Topics |
| WOS Subject | Multidisciplinary Sciences |
| Funding Organization | National Key Research and Development Program of China ; Basic Frontier Scientific Research Program of Chinese Academy of Sciences ; Foundation for Innovative Research Groups of the National Natural Science Foundation of China |
| WOS ID | WOS:000909465300041 |
| Publisher | JOURNAL OF VISUALIZED EXPERIMENTS |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.ipe.ac.cn/handle/122111/56464 |
| Collection | 中国科学院过程工程研究所 |
| Corresponding Author | Ogino, Kenji |
| Affiliation | 1.Tokyo Univ Agr & Technol, Grad Sch Bio Applicat & Syst Engn, Tokyo, Japan 2.Tokyo Univ Agr & Technol, Inst Global Innovat Res, Tokyo, Japan 3.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing, Peoples R China 4.Univ Chinese Acad Sci, Beijing, Peoples R China |
| Recommended Citation GB/T 7714 | Cao, Fengqiang,Ming, Yali,Gao, Weixiang,et al. Cellular Affinity of Particle-Stabilized Emulsion to Boost Antigen Internalization[J]. JOVE-JOURNAL OF VISUALIZED EXPERIMENTS,2022(187):18. |
| APA | Cao, Fengqiang,Ming, Yali,Gao, Weixiang,Ge, Jia,&Ogino, Kenji.(2022).Cellular Affinity of Particle-Stabilized Emulsion to Boost Antigen Internalization.JOVE-JOURNAL OF VISUALIZED EXPERIMENTS(187),18. |
| MLA | Cao, Fengqiang,et al."Cellular Affinity of Particle-Stabilized Emulsion to Boost Antigen Internalization".JOVE-JOURNAL OF VISUALIZED EXPERIMENTS .187(2022):18. |
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