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| Electrolytic stimulation in aid of poly(beta-L-malic acid) production by Aureobasidium melanogenum ipe-1 | |
| Cao, Weifeng1,2; Deng, Tingting3; Shen, Fei1,2; Rong, Fan1,2; Qiao, Changsheng4; Wan, Yinhua1,2 | |
| 2022-12-31 | |
| Source Publication | INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
 |
| ISSN | 0141-8130 |
| Volume | 223Pages:722-731 |
| Abstract | Poly (beta-L-malic acid) (PMLA) is attracting industrial interest for its potential application in medicine and other industries. In this study, electrolytic stimulation assisted PMLA production was developed. Firstly, it was found that the pentavalent nitrogen source (i.e., NO3-) was more suitable for PMLA production. Secondly, a usual single-chamber bioelectric-fermentation system (BES) cannot improve PMLA production, which can only promote cell growth. Then, a new single-chamber BES with an external circulation was developed, where the PMLA metabolism was further intensified. Finally, the integration of NO3- addition and electrolytic stimulation mode (c) showed a positive synergy on the PMLA production. Compared to the case without NO3- addition and electrolytic stimulation, the PMLA production was increased by 22.9 % using the integrated process. Moreover, compared to the case without the electrolytic stimulation mode (c), it was revealed that the different genes involved in 12 metabolic subsystems using the integrated process, where 31 and 177 genes were up-regulated and down-regulated, respectively. The up-regulated genes were mainly participated in melanin metabolic process, catalase activity, and oxidoreductase activity. Hence, the integration of electrolytic stimulation represents a novel approach to improve PMLA production. |
| Keyword | Poly(beta-L-malic acid) Aureobasidium melanogenum Electrolytic stimulation Bioelectric-fermentation system Oxidation-reduction potential |
| DOI | 10.1016/j.ijbiomac.2022.11.026 |
| Language | 英语 |
| WOS Keyword | CORYNEBACTERIUM-GLUTAMICUM ; MICROBIAL ELECTROSYNTHESIS ; ANTIOXIDANT ACTIVITY ; NEUTRAL RED ; PULLULANS ; BIOSYNTHESIS ; FERMENTATION ; MELANIN |
| Funding Project | National Natural Science Foundation of China, China ; National Key R & D Program of China, China ; Beijing Natural Science Foundation, China ; [22278412] ; [2021YFF0601903] ; [8202044] |
| WOS Research Area | Biochemistry & Molecular Biology ; Chemistry ; Polymer Science |
| WOS Subject | Biochemistry & Molecular Biology ; Chemistry, Applied ; Polymer Science |
| Funding Organization | National Natural Science Foundation of China, China ; National Key R & D Program of China, China ; Beijing Natural Science Foundation, China |
| WOS ID | WOS:000906838700004 |
| Publisher | ELSEVIER |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.ipe.ac.cn/handle/122111/56480 |
| Collection | 中国科学院过程工程研究所 |
| Corresponding Author | Cao, Weifeng; Wan, Yinhua |
| Affiliation | 1.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing 100190, Peoples R China 2.Univ Chinese Acad Sci, Sch Chem Engn, Beijing 100049, Peoples R China 3.Chinese Acad Inspection & Quarantine, Agroprod Safety Res Ctr, Beijing 100123, Peoples R China 4.Tianjin Univ Sci & Technol, Coll Biotechnol, Tianjin 300457, Peoples R China |
| Recommended Citation GB/T 7714 | Cao, Weifeng,Deng, Tingting,Shen, Fei,et al. Electrolytic stimulation in aid of poly(beta-L-malic acid) production by Aureobasidium melanogenum ipe-1[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,2022,223:722-731. |
| APA | Cao, Weifeng,Deng, Tingting,Shen, Fei,Rong, Fan,Qiao, Changsheng,&Wan, Yinhua.(2022).Electrolytic stimulation in aid of poly(beta-L-malic acid) production by Aureobasidium melanogenum ipe-1.INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,223,722-731. |
| MLA | Cao, Weifeng,et al."Electrolytic stimulation in aid of poly(beta-L-malic acid) production by Aureobasidium melanogenum ipe-1".INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 223(2022):722-731. |
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