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Modeling of the mixed culture and periodic control for PHB production
Alternative TitleBiochem. Eng. J.
Tohyama, M; Patarinska, T; Qiang, ZW; Shimizu, K
2002-04-01
Source PublicationBIOCHEMICAL ENGINEERING JOURNAL
ISSN1369-703X
Volume10Issue:3Pages:157-173
AbstractThe unstructured mathematical model was developed in the present investigation for the mixed culture, where the metabolites produced by one microorganism is assimilated by the other microorganism. For this, we specifically employed such model system in which sugars such as glucose were converted to lactate by Lactobacillus delbrueckii and the lactate was converted in turn to poly-beta-hydroxybutyrate (PHB) by Ralstonia eutropha in one fermentor. Several batch and fed-batch culture experiments were conducted using each microorganism at different dissolved oxygen (DO) concentrations. Those experimental data were then fitted to the mathematical model, which can describe the dynamics of a mixed culture. Some of the model parameters were expressed as functions of DO concentrations, and some of the other model parameters were tuned based on the mixed culture experiments. The model developed describes the effects of such concentrations of glucose, lactate, DO, and NH3 on the dynamic behavior of such concentrations as both microorganisms, glucose, lactate, and PHB. Optimal operating condition was then investigated using the model developed, It was found that the periodic change in DO concentration improved such performance as PHB yield, and it was verified by experiments. The optimal NH3 concentration profile was also obtained for the efficient PHB production by the application of the maximum principle, (C) 2002 Elsevier Science B.V. All rights reserved.; The unstructured mathematical model was developed in the present investigation for the mixed culture, where the metabolites produced by one microorganism is assimilated by the other microorganism. For this, we specifically employed such model system in which sugars such as glucose were converted to lactate by Lactobacillus delbrueckii and the lactate was converted in turn to poly-beta-hydroxybutyrate (PHB) by Ralstonia eutropha in one fermentor. Several batch and fed-batch culture experiments were conducted using each microorganism at different dissolved oxygen (DO) concentrations. Those experimental data were then fitted to the mathematical model, which can describe the dynamics of a mixed culture. Some of the model parameters were expressed as functions of DO concentrations, and some of the other model parameters were tuned based on the mixed culture experiments. The model developed describes the effects of such concentrations of glucose, lactate, DO, and NH3 on the dynamic behavior of such concentrations as both microorganisms, glucose, lactate, and PHB. Optimal operating condition was then investigated using the model developed, It was found that the periodic change in DO concentration improved such performance as PHB yield, and it was verified by experiments. The optimal NH3 concentration profile was also obtained for the efficient PHB production by the application of the maximum principle, (C) 2002 Elsevier Science B.V. All rights reserved.
KeywordMixed Culture Phb Fermentation Dynamics Modeling Periodic Operation
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Technology
URL查看原文
Indexed BySCI
Language英语
WOS KeywordBETA-HYDROXYBUTYRIC ACID ; LACTOCOCCUS-LACTIS IO-1 ; ALCALIGENES-EUTROPHUS ; LACTOBACILLUS-DELBRUECKII ; POLY(BETA-HYDROXYBUTYRIC ACID) ; SACCHAROMYCES-CEREVISIAE ; FERMENTATIVE PRODUCTION ; MATHEMATICAL-MODEL ; RALSTONIA-EUTROPHA ; COCULTURE SYSTEM
WOS Research AreaBiotechnology & Applied Microbiology ; Engineering
WOS SubjectBiotechnology & Applied Microbiology ; Engineering, Chemical
WOS IDWOS:000175027100001
Citation statistics
Cited Times:40[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Version出版稿
Identifierhttp://ir.ipe.ac.cn/handle/122111/5649
Collection研究所(批量导入)
Affiliation1.Kyushu Inst Technol, Dept Biochem Engn, Fukuoka 8200067, Japan
2.Bulgarian Acad Sci, Inst Control & Syst Res, Dept Adapt & Robust Control, Sofia, Bulgaria
3.Chinese Acad Sci, Inst Chem Met, State Key Lab Biochem Engn, Beijing, Peoples R China
Recommended Citation
GB/T 7714
Tohyama, M,Patarinska, T,Qiang, ZW,et al. Modeling of the mixed culture and periodic control for PHB production[J]. BIOCHEMICAL ENGINEERING JOURNAL,2002,10(3):157-173.
APA Tohyama, M,Patarinska, T,Qiang, ZW,&Shimizu, K.(2002).Modeling of the mixed culture and periodic control for PHB production.BIOCHEMICAL ENGINEERING JOURNAL,10(3),157-173.
MLA Tohyama, M,et al."Modeling of the mixed culture and periodic control for PHB production".BIOCHEMICAL ENGINEERING JOURNAL 10.3(2002):157-173.
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