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Cloning, expression and purification of gussurobin, a thrombin-like enzyme from the snake venom of Gloydius ussuriensis
Alternative TitleActa Biochim. Biophys. Sin.
Yang, Q; Hu, XJ; Xu, XM; An, LJ; Yuan, XD; Su, ZG; Janson, JC
2002
Source PublicationACTA BIOCHIMICA ET BIOPHYSICA SINICA
ISSN0582-9879
Volume34Issue:1Pages:6-10
AbstractTotal RNAs were extracted from the venom gland of the snake Gloydius ussuriensis. The cDNA of gussurobin, a thrombin-like enzyme from Gloydius ussuriensis, was cloned and amplified by RT-PCR. Assay of the nucleotide sequence of the cDNA allowed postulation of the complete amino acid sequence for Gloydius ussuriensis, Chinese Viperdae. Its amino acid sequence exhibits significant homology with that of other snake thrombin-like enzymes. The cDNA of gussurobin was inserted into the vector pPIC 9K and expressed successfully in Pichia pastoris, strain GS115. The recombinant gussurobin was separated and purified from 500 ml culture and showed one band on SDS-PAGE.; Total RNAs were extracted from the venom gland of the snake Gloydius ussuriensis. The cDNA of gussurobin, a thrombin-like enzyme from Gloydius ussuriensis, was cloned and amplified by RT-PCR. Assay of the nucleotide sequence of the cDNA allowed postulation of the complete amino acid sequence for Gloydius ussuriensis, Chinese Viperdae. Its amino acid sequence exhibits significant homology with that of other snake thrombin-like enzymes. The cDNA of gussurobin was inserted into the vector pPIC 9K and expressed successfully in Pichia pastoris, strain GS115. The recombinant gussurobin was separated and purified from 500 ml culture and showed one band on SDS-PAGE.
KeywordThrombin-like Enzyme Gussurobin Cdna Sequence Expression Purification
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
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Indexed BySCI
Language英语
WOS KeywordMOLECULAR-CLONING ; SEQUENCE-ANALYSIS ; PICHIA-PASTORIS ; CDNA ; RHODOSTOMA ; BATROXOBIN ; PROTEASES ; ANCROD ; ACID
WOS Research AreaBiochemistry & Molecular Biology ; Biophysics
WOS SubjectBiochemistry & Molecular Biology ; Biophysics
WOS IDWOS:000173426700002
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Cited Times:7[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Version出版稿
Identifierhttp://ir.ipe.ac.cn/handle/122111/5664
Collection研究所(批量导入)
Affiliation1.Dalian Univ Technol, Bioengn Inst, Dalian 116012, Peoples R China
2.Takara Biotech Dalian Co Ltd, Dalian 116600, Peoples R China
3.Chinese Acad Sci, State Key Lab Biochem Engn, Inst Chem Met, Beijing 100080, Peoples R China
4.Biomed Ctr 577, Biosurface Sci Ctr, S-75123 Uppsala, Sweden
Recommended Citation
GB/T 7714
Yang, Q,Hu, XJ,Xu, XM,et al. Cloning, expression and purification of gussurobin, a thrombin-like enzyme from the snake venom of Gloydius ussuriensis[J]. ACTA BIOCHIMICA ET BIOPHYSICA SINICA,2002,34(1):6-10.
APA Yang, Q.,Hu, XJ.,Xu, XM.,An, LJ.,Yuan, XD.,...&Janson, JC.(2002).Cloning, expression and purification of gussurobin, a thrombin-like enzyme from the snake venom of Gloydius ussuriensis.ACTA BIOCHIMICA ET BIOPHYSICA SINICA,34(1),6-10.
MLA Yang, Q,et al."Cloning, expression and purification of gussurobin, a thrombin-like enzyme from the snake venom of Gloydius ussuriensis".ACTA BIOCHIMICA ET BIOPHYSICA SINICA 34.1(2002):6-10.
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