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Cloning and expression of defibrase cDNA from the venom of Gloydius shedaoensis
Alternative TitleBiotechnol. Lett.
Yang, Q; Xu, XM; Li, M; Yuan, XD; Su, ZG; Janson, JC; An, LJ
2002
Source PublicationBIOTECHNOLOGY LETTERS
ISSN0141-5492
Volume24Issue:2Pages:135-138
AbstractThe total RNA was extracted from the venom gland of snake, Gloydius shedaoensis. The defibrase cDNA was amplified by RT-PCR. Assaying the nucleotide sequence of the cDNA allowed the complete amino acid sequence for defibrase of Gloydius shedaoensis to be determined. The defibrase cDNA was inserted into the vector, pPIC 9K, and expressed in Pichia pastoris which then produced 10 mg target protein l(-1).; The total RNA was extracted from the venom gland of snake, Gloydius shedaoensis. The defibrase cDNA was amplified by RT-PCR. Assaying the nucleotide sequence of the cDNA allowed the complete amino acid sequence for defibrase of Gloydius shedaoensis to be determined. The defibrase cDNA was inserted into the vector, pPIC 9K, and expressed in Pichia pastoris which then produced 10 mg target protein l(-1).
KeywordCdna Sequence Defibrase Expression Separation Thrombin-like Enzyme
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
URL查看原文
Indexed BySCI
Language英语
WOS KeywordTHROMBIN-LIKE ENZYME ; SEQUENCE-ANALYSIS
WOS Research AreaBiotechnology & Applied Microbiology
WOS SubjectBiotechnology & Applied Microbiology
WOS IDWOS:000173417300008
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Cited Times:10[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Version出版稿
Identifierhttp://ir.ipe.ac.cn/handle/122111/5666
Collection研究所(批量导入)
Affiliation1.Dalian Univ Technol, Bioengn Inst, Dalian 116012, Peoples R China
2.Takara Biotech Dalian Co Ltd, Dalian 116600, Peoples R China
3.Chinese Acad Sci, Inst Chem Met, State Key Lab Biochem Engn, Beijing 100080, Peoples R China
4.Biomed Ctr, Biosurface Sci Ctr, S-75123 Uppsala, Sweden
Recommended Citation
GB/T 7714
Yang, Q,Xu, XM,Li, M,et al. Cloning and expression of defibrase cDNA from the venom of Gloydius shedaoensis[J]. BIOTECHNOLOGY LETTERS,2002,24(2):135-138.
APA Yang, Q.,Xu, XM.,Li, M.,Yuan, XD.,Su, ZG.,...&An, LJ.(2002).Cloning and expression of defibrase cDNA from the venom of Gloydius shedaoensis.BIOTECHNOLOGY LETTERS,24(2),135-138.
MLA Yang, Q,et al."Cloning and expression of defibrase cDNA from the venom of Gloydius shedaoensis".BIOTECHNOLOGY LETTERS 24.2(2002):135-138.
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