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Selective precipitation of water-soluble proteins using designed polyelectrolyte
Alternative TitleSep. Sci. Technol.
Yu, J; Liu, HZ; Chen, JY
2002
Source PublicationSEPARATION SCIENCE AND TECHNOLOGY
ISSN0149-6395
Volume37Issue:1Pages:217-228
AbstractBy using lysozyme, hemoglobin (bovine), and pepsin as the model proteins and copolymer F1110 containing reactive functional groups of amido, sulfonate, and carboxylic acid groups as the flocculating agents, selective precipitation of proteins was performed by using two chemical processes. The experiment shows that the surface charge of copolymer F1110 is closely related to the pH value in solution. The flocculation behaviors of proteins with F1110 can be controlled completely by adjusting pH value in solution, which realizes the selective separation of mixed proteins. In addition, the selective precipitation of the proteins is promoted by a "modified flocculation" process, in two steps with a combination of F1110 and polyacrylamide. The characterization of modified flocculation is closely related to the property of the protein. These studies show a great prospect for selectively separating proteins from a mixture solution using designed polyelectrolytes containing reactive functional groups.; By using lysozyme, hemoglobin (bovine), and pepsin as the model proteins and copolymer F1110 containing reactive functional groups of amido, sulfonate, and carboxylic acid groups as the flocculating agents, selective precipitation of proteins was performed by using two chemical processes. The experiment shows that the surface charge of copolymer F1110 is closely related to the pH value in solution. The flocculation behaviors of proteins with F1110 can be controlled completely by adjusting pH value in solution, which realizes the selective separation of mixed proteins. In addition, the selective precipitation of the proteins is promoted by a "modified flocculation" process, in two steps with a combination of F1110 and polyacrylamide. The characterization of modified flocculation is closely related to the property of the protein. These studies show a great prospect for selectively separating proteins from a mixture solution using designed polyelectrolytes containing reactive functional groups.
KeywordWater-soluble Protein Polyelectrolyte Flocculation Selective Precipitation
SubtypeArticle
WOS HeadingsScience & Technology ; Physical Sciences ; Technology
URL查看原文
Indexed BySCI
Language英语
WOS KeywordPOLYMER MOLECULAR-WEIGHT ; FLOCCULATION ; POLYETHYLENEIMINE ; COACERVATION ; ADSORPTION ; SEPARATION ; COPOLYMER ; BEHAVIOR ; RATIO
WOS Research AreaChemistry ; Engineering
WOS SubjectChemistry, Multidisciplinary ; Engineering, Chemical
WOS IDWOS:000173792700012
Citation statistics
Cited Times:1[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Version出版稿
Identifierhttp://ir.ipe.ac.cn/handle/122111/5668
Collection研究所(批量导入)
AffiliationChinese Acad Sci, Inst Chem Met, Young Scientist Lab Separat Sci & Engn, Beijing 100080, Peoples R China
Recommended Citation
GB/T 7714
Yu, J,Liu, HZ,Chen, JY. Selective precipitation of water-soluble proteins using designed polyelectrolyte[J]. SEPARATION SCIENCE AND TECHNOLOGY,2002,37(1):217-228.
APA Yu, J,Liu, HZ,&Chen, JY.(2002).Selective precipitation of water-soluble proteins using designed polyelectrolyte.SEPARATION SCIENCE AND TECHNOLOGY,37(1),217-228.
MLA Yu, J,et al."Selective precipitation of water-soluble proteins using designed polyelectrolyte".SEPARATION SCIENCE AND TECHNOLOGY 37.1(2002):217-228.
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