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Mechanisms of protein fouling in microfiltration. I. Determination of proteins fouled on microfiltration membranes
Alternative TitleSep. Sci. Technol.
Zhang, JD; Cai, ZL; Cong, W; Su, ZG; Fan, OY
2002
Source PublicationSEPARATION SCIENCE AND TECHNOLOGY
ISSN0149-6395
Volume37Issue:13Pages:3025-3038
AbstractProtein fouling is one of the critical factors governing the effectiveness of many microfiltration processes. Although mechanisms have been proposed, direct correlation between protein amount fouled on membranes and filtration behavior is required. We developed a simple method to measure proteins on microfiltration membranes. The protein-fouled membranes were stained by amido black 10B first, then destained to remove excess dye not bound to protein. Finally, the dye associated with the protein on the membranes was eluted with 0.1 N NaOH. Three membranes (mixed esters of cellulose nitrate and acetate membrane, Durapore GVWP membrane, and. nuclear-pore membrane) were examined by blotting, adsorbing, and depositing bovine serum albumin on them. The absorbency of the eluted solutions was measured at 620 nm. The absorbency of the eluted solutions was independent of staining time and eluting time, but decreased with prolonged destaining time. The results showed a good linear relationship between the absorbency and amount of protein on membranes in all conditions examined. Three other proteins were also examined and the results showed that different proteins have different slopes. These results indicate that the method could be used to quantify proteins fouled on membrane.; Protein fouling is one of the critical factors governing the effectiveness of many microfiltration processes. Although mechanisms have been proposed, direct correlation between protein amount fouled on membranes and filtration behavior is required. We developed a simple method to measure proteins on microfiltration membranes. The protein-fouled membranes were stained by amido black 10B first, then destained to remove excess dye not bound to protein. Finally, the dye associated with the protein on the membranes was eluted with 0.1 N NaOH. Three membranes (mixed esters of cellulose nitrate and acetate membrane, Durapore GVWP membrane, and. nuclear-pore membrane) were examined by blotting, adsorbing, and depositing bovine serum albumin on them. The absorbency of the eluted solutions was measured at 620 nm. The absorbency of the eluted solutions was independent of staining time and eluting time, but decreased with prolonged destaining time. The results showed a good linear relationship between the absorbency and amount of protein on membranes in all conditions examined. Three other proteins were also examined and the results showed that different proteins have different slopes. These results indicate that the method could be used to quantify proteins fouled on membrane.
KeywordQuantification Protein Fouling Microfiltration
SubtypeArticle
WOS HeadingsScience & Technology ; Physical Sciences ; Technology
URL查看原文
Indexed BySCI
Language英语
WOS KeywordULTRAFILTRATION MEMBRANES ; ADSORPTION ; BEHAVIOR ; BSA
WOS Research AreaChemistry ; Engineering
WOS SubjectChemistry, Multidisciplinary ; Engineering, Chemical
WOS IDWOS:000178523600004
Citation statistics
Cited Times:3[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Version出版稿
Identifierhttp://ir.ipe.ac.cn/handle/122111/5679
Collection研究所(批量导入)
AffiliationChinese Acad Sci, Inst Proc Engn, Natl Lab Biochem Engn China, Beijing 100080, Peoples R China
Recommended Citation
GB/T 7714
Zhang, JD,Cai, ZL,Cong, W,et al. Mechanisms of protein fouling in microfiltration. I. Determination of proteins fouled on microfiltration membranes[J]. SEPARATION SCIENCE AND TECHNOLOGY,2002,37(13):3025-3038.
APA Zhang, JD,Cai, ZL,Cong, W,Su, ZG,&Fan, OY.(2002).Mechanisms of protein fouling in microfiltration. I. Determination of proteins fouled on microfiltration membranes.SEPARATION SCIENCE AND TECHNOLOGY,37(13),3025-3038.
MLA Zhang, JD,et al."Mechanisms of protein fouling in microfiltration. I. Determination of proteins fouled on microfiltration membranes".SEPARATION SCIENCE AND TECHNOLOGY 37.13(2002):3025-3038.
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