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Forward and backward extraction of BSA using mixed reverse micellar system of CTAB and alkyl halides
Alternative TitleBiochem. Eng. J.
Zhang, W; Liu, HZ; Chen, JY
2002-10-01
Source PublicationBIOCHEMICAL ENGINEERING JOURNAL
ISSN1369-703X
Volume12Issue:1Pages:1-5
AbstractThe forward and backward extraction of bovine serum albumin (BSA) has been studied using mixed reverse micellar systems of cetyl trimethyl ammoniumbromide (CTAB) and alkyl halides (including 1-chlorobutane (R4Cl), 1-bromooctane (R8Br) and 1-iodobutane (R4I)). The addition of alkyl halide R8Br to 50 mmolA CTAB/20% (v/v) hexanol/petroleurn ether reverse micellar system could improve the BSA transfer from the aqueous phase to reverse micellar phase, while the addition of R4Cl or R4I had almost no effect on the BSA transfer. The mixed reverse micelles formed with CTAB and alkyl halides exhibit excellent backward extraction behavior for BSA. The mixed reverse micelles formed with CTAB and R4I can realize the recovery of BSA effectively in a wide range of pH up to or higher than the isoclectric point of BSA. The mixed reverse trucelles formed with CTAB and R4Cl, R8Br or R4I can obviously enhance the BSA backward transfer at low ionic strength with addition of KBr or KCl as electrolyte. The mixed reverse micellar system indicates that it requires less time to reach the mass transfer equilibrium in comparison with the reverse micellar system with CTAB only. The mechanism of backward extraction proposed that with the addition of alkyl halides to CTAB reverse micelles, the hydrophobic interaction between the reverse micelles decreased. (C) 2002 Elsevier Science B.V. All rights reserved.; The forward and backward extraction of bovine serum albumin (BSA) has been studied using mixed reverse micellar systems of cetyl trimethyl ammoniumbromide (CTAB) and alkyl halides (including 1-chlorobutane (R4Cl), 1-bromooctane (R8Br) and 1-iodobutane (R4I)). The addition of alkyl halide R8Br to 50 mmolA CTAB/20% (v/v) hexanol/petroleurn ether reverse micellar system could improve the BSA transfer from the aqueous phase to reverse micellar phase, while the addition of R4Cl or R4I had almost no effect on the BSA transfer. The mixed reverse micelles formed with CTAB and alkyl halides exhibit excellent backward extraction behavior for BSA. The mixed reverse micelles formed with CTAB and R4I can realize the recovery of BSA effectively in a wide range of pH up to or higher than the isoclectric point of BSA. The mixed reverse trucelles formed with CTAB and R4Cl, R8Br or R4I can obviously enhance the BSA backward transfer at low ionic strength with addition of KBr or KCl as electrolyte. The mixed reverse micellar system indicates that it requires less time to reach the mass transfer equilibrium in comparison with the reverse micellar system with CTAB only. The mechanism of backward extraction proposed that with the addition of alkyl halides to CTAB reverse micelles, the hydrophobic interaction between the reverse micelles decreased. (C) 2002 Elsevier Science B.V. All rights reserved.
KeywordReverse Micelles Backward Extraction Alkyl Halide Bsa Ctab
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Technology
URL查看原文
Indexed BySCI
Language英语
WOS KeywordPHOSPHORIC-ACID ; AMINO-ACIDS ; PROTEINS ; RECOVERY ; SULFOSUCCINATE ; WATER ; DYE
WOS Research AreaBiotechnology & Applied Microbiology ; Engineering
WOS SubjectBiotechnology & Applied Microbiology ; Engineering, Chemical
WOS IDWOS:000178450600001
Citation statistics
Cited Times:20[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Version出版稿
Identifierhttp://ir.ipe.ac.cn/handle/122111/5684
Collection研究所(批量导入)
AffiliationChinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Young Scientist Lab Separat Sci & Engn, Beijing 100080, Peoples R China
Recommended Citation
GB/T 7714
Zhang, W,Liu, HZ,Chen, JY. Forward and backward extraction of BSA using mixed reverse micellar system of CTAB and alkyl halides[J]. BIOCHEMICAL ENGINEERING JOURNAL,2002,12(1):1-5.
APA Zhang, W,Liu, HZ,&Chen, JY.(2002).Forward and backward extraction of BSA using mixed reverse micellar system of CTAB and alkyl halides.BIOCHEMICAL ENGINEERING JOURNAL,12(1),1-5.
MLA Zhang, W,et al."Forward and backward extraction of BSA using mixed reverse micellar system of CTAB and alkyl halides".BIOCHEMICAL ENGINEERING JOURNAL 12.1(2002):1-5.
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