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Catalysis mechanism to increase taxol from the extract of Taxus cuspidate callus cultures with alumina chromatography
Alternative TitleSep. Sci. Technol.
Zhang, ZQ; Su, ZG
2002
Source PublicationSEPARATION SCIENCE AND TECHNOLOGY
ISSN0149-6395
Volume37Issue:3Pages:733-743
AbstractIn our previous research, we had reported a chromatographic protocol using basic Al2O3 column to separate and recover taxol from the extract of Taxus cuspidate callus cultures. On the basis of the amount of taxol detected in the starting materials of the crude plant extract, the recovery of taxol was more than 170% after the basic Al2O3 column chromatography step. It was found that the main source of taxol increase was 7-epi-taxol in the crude extract conversion to taxol by catalysis of the Al2O3 Medium, In this paper, the catalysis mechanism of Al2O3 in converting 7-epi-taxol to taxol was developed by investigating the surface structure of alumina and the formation of activity core of catalysis. It was found that the type of Al2O3, the content of Al2O3 in the stationary phase, and the concentration of H2O in the mobile phase of chromatography could influence the isomeric reaction. The isomerization of 7-epi-taxol to taxol was under the influence of Lewis souci and basic activity cores on the surface of alumina. The strength and type of activity core controlled the procedure of reaction.; In our previous research, we had reported a chromatographic protocol using basic Al2O3 column to separate and recover taxol from the extract of Taxus cuspidate callus cultures. On the basis of the amount of taxol detected in the starting materials of the crude plant extract, the recovery of taxol was more than 170% after the basic Al2O3 column chromatography step. It was found that the main source of taxol increase was 7-epi-taxol in the crude extract conversion to taxol by catalysis of the Al2O3 Medium, In this paper, the catalysis mechanism of Al2O3 in converting 7-epi-taxol to taxol was developed by investigating the surface structure of alumina and the formation of activity core of catalysis. It was found that the type of Al2O3, the content of Al2O3 in the stationary phase, and the concentration of H2O in the mobile phase of chromatography could influence the isomeric reaction. The isomerization of 7-epi-taxol to taxol was under the influence of Lewis souci and basic activity cores on the surface of alumina. The strength and type of activity core controlled the procedure of reaction.
KeywordAlumina Catalysis 7-epi-taxol Mechanism Taxol
SubtypeArticle
WOS HeadingsScience & Technology ; Physical Sciences ; Technology
URL查看原文
Indexed BySCI
Language英语
WOS KeywordBREVIFOLIA ; TAXANES ; PHASE
WOS Research AreaChemistry ; Engineering
WOS SubjectChemistry, Multidisciplinary ; Engineering, Chemical
WOS IDWOS:000174241300014
Citation statistics
Cited Times:2[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Version出版稿
Identifierhttp://ir.ipe.ac.cn/handle/122111/5688
Collection研究所(批量导入)
AffiliationChinese Acad Sci, State Key Lab Biochem Engn, Inst Chem Met, Beijing 100080, Peoples R China
Recommended Citation
GB/T 7714
Zhang, ZQ,Su, ZG. Catalysis mechanism to increase taxol from the extract of Taxus cuspidate callus cultures with alumina chromatography[J]. SEPARATION SCIENCE AND TECHNOLOGY,2002,37(3):733-743.
APA Zhang, ZQ,&Su, ZG.(2002).Catalysis mechanism to increase taxol from the extract of Taxus cuspidate callus cultures with alumina chromatography.SEPARATION SCIENCE AND TECHNOLOGY,37(3),733-743.
MLA Zhang, ZQ,et al."Catalysis mechanism to increase taxol from the extract of Taxus cuspidate callus cultures with alumina chromatography".SEPARATION SCIENCE AND TECHNOLOGY 37.3(2002):733-743.
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