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An automatic system for multidimensional integrated protein chromatography
Kong, Yingjun1,2; Li, Xiunan1; Bai, Gaoying3; Ma, Guanghui1; Su, Zhiguo1; Li, XN
2010-10-29
Source PublicationJOURNAL OF CHROMATOGRAPHY A
ISSN0021-9673
Volume1217Issue:44Pages:6898-6904
AbstractAn automatic system for multidimensional integrated protein chromatography was designed for simultaneous separation of multiple proteins from complex mixtures, such as human plasma and tissue lysates. This computer-controlled system integrates several chromatographic columns that work independently or cooperatively with one another to achieve efficient high throughputs. The pipelines can be automatically switched either to another column or to a collection container for each UV-detected elution fraction. Environmental contamination is avoided due to the closed fluid paths and elimination of manual column change. This novel system was successfully used for simultaneous preparation of five proteins from the precipitate of human plasma fraction IV (fraction IV). The system involved gel filtration, ion exchange, hydrophobic interaction, and heparin affinity chromatography. Human serum albumin (HSA), transferrin (TO, antithrombin-III (AT-III), alpha 1-antitrypsin (alpha 1-AT), and haptoglobin (Hp) were purified within 3 h. The following recovery and purity were achieved: 95% (RSD, 2.8%) and 95% for HSA, 80% (RSD, 2.0%) and 99% for Tf, 70% (RSD, 2.1%) and 99% for AT-III, 65% (RSD, 2.0%) and 94% for alpha 1-AT, and 50% (RSD, 1.0%) and 90% for Hp. The results demonstrate that this novel multidimensional integrated chromatography system is capable of simultaneously separating multiple protein products from the same raw material with high yield and purity and it has the potential for a wide range of multi-step chromatography separation processes. (c) 2010 Elsevier B.V. All rights reserved.
KeywordMultidimensional Biorefinery Chromatography Protein Purification
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Physical Sciences
DOI10.1016/j.chroma.2010.08.067
Indexed BySCI
Language英语
WOS KeywordANTIBODY AFFINITY-CHROMATOGRAPHY ; LIQUID-CHROMATOGRAPHY ; MILLIGRAM QUANTITIES ; ANTITHROMBIN-III ; HAPTOGLOBIN 1-1 ; PURIFICATION ; IDENTIFICATION ; TRANSFERRIN ; STRATEGIES ; SERUM
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry
WOS SubjectBiochemical Research Methods ; Chemistry, Analytical
WOS IDWOS:000283804800012
Citation statistics
Cited Times:10[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Version出版稿
Identifierhttp://ir.ipe.ac.cn/handle/122111/5690
Collection生化工程国家重点实验室
Corresponding AuthorLi, XN
Affiliation1.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China
2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
3.Lanzhou Univ, Sch Life Sci, Lanzhou 730000, Peoples R China
Recommended Citation
GB/T 7714
Kong, Yingjun,Li, Xiunan,Bai, Gaoying,et al. An automatic system for multidimensional integrated protein chromatography[J]. JOURNAL OF CHROMATOGRAPHY A,2010,1217(44):6898-6904.
APA Kong, Yingjun,Li, Xiunan,Bai, Gaoying,Ma, Guanghui,Su, Zhiguo,&Li, XN.(2010).An automatic system for multidimensional integrated protein chromatography.JOURNAL OF CHROMATOGRAPHY A,1217(44),6898-6904.
MLA Kong, Yingjun,et al."An automatic system for multidimensional integrated protein chromatography".JOURNAL OF CHROMATOGRAPHY A 1217.44(2010):6898-6904.
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