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Fluorescence investigation of affinity interaction between bovine serum albumin and triazine dye in reversed micelles
Alternative TitleAppl. Biochem. Biotechnol.
Zhang, TX; Liu, HZ; Chen, JY
2001-09-01
Source PublicationAPPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
ISSN0273-2289
Volume95Issue:3Pages:163-174
AbstractThe effect of Cibacron Blue 3GA (CB) on the fluorescence emission spectra of bovine serum albumin (BSA) was investigated in cationic reversed micelles formed with cetyltrimethylammonium bromide (CTAB) compared with that in aqueous phase. The anionic CB had electrostatic interactions with cationic CTAB and affinity interactions with BSA in the reversed micelles. The addition of CB in the reversed micellar phase led to a great decrease in the fluorescence intensity of BSA and a remarkable red shift of the wavelength of emission maximum (lambda (max)). The fluorescence intensity of BSA decreased and the lambda (max) decreased 5 nm (blue shift) without the addition of CB in the reversed micellar phase. The fluorescence intensity of BSA with the addition of CB had the strongest value in the aqueous phase with the presence of CTAB, a less strong value in the reversed micellar phase, and a weak value in the aqueous phase without the presence of CTAB. The increase in X max of BSA with the addition of CB in the reversed micellar phase might indicate the decrease in the hydrophobic microenvironment of the Trp residue of BSA, contrary to those microenvironments in the absence of CB.; The effect of Cibacron Blue 3GA (CB) on the fluorescence emission spectra of bovine serum albumin (BSA) was investigated in cationic reversed micelles formed with cetyltrimethylammonium bromide (CTAB) compared with that in aqueous phase. The anionic CB had electrostatic interactions with cationic CTAB and affinity interactions with BSA in the reversed micelles. The addition of CB in the reversed micellar phase led to a great decrease in the fluorescence intensity of BSA and a remarkable red shift of the wavelength of emission maximum (lambda (max)). The fluorescence intensity of BSA decreased and the lambda (max) decreased 5 nm (blue shift) without the addition of CB in the reversed micellar phase. The fluorescence intensity of BSA with the addition of CB had the strongest value in the aqueous phase with the presence of CTAB, a less strong value in the reversed micellar phase, and a weak value in the aqueous phase without the presence of CTAB. The increase in X max of BSA with the addition of CB in the reversed micellar phase might indicate the decrease in the hydrophobic microenvironment of the Trp residue of BSA, contrary to those microenvironments in the absence of CB.
KeywordAffinity Interaction Reversed Micelles Bovine Serum Albumin Cibacron Blue 3ga Fluorescence Emission Spectra
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
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Indexed BySCI
Language英语
WOS KeywordMYELIN BASIC-PROTEIN ; NONIONIC SURFACTANT ; CIBACRON BLUE ; EXTRACTION ; BIOAFFINITY ; SEPARATION ; TRYPSIN ; DYNAMICS ; SYSTEMS ; ENZYMES
WOS Research AreaBiochemistry & Molecular Biology ; Biotechnology & Applied Microbiology
WOS SubjectBiochemistry & Molecular Biology ; Biotechnology & Applied Microbiology
WOS IDWOS:000171816400002
Citation statistics
Cited Times:3[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Version出版稿
Identifierhttp://ir.ipe.ac.cn/handle/122111/5744
Collection研究所(批量导入)
AffiliationChinese Acad Sci, Inst Chem Met, Lab Separat Sci & Engn, Beijing 100080, Peoples R China
Recommended Citation
GB/T 7714
Zhang, TX,Liu, HZ,Chen, JY. Fluorescence investigation of affinity interaction between bovine serum albumin and triazine dye in reversed micelles[J]. APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY,2001,95(3):163-174.
APA Zhang, TX,Liu, HZ,&Chen, JY.(2001).Fluorescence investigation of affinity interaction between bovine serum albumin and triazine dye in reversed micelles.APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY,95(3),163-174.
MLA Zhang, TX,et al."Fluorescence investigation of affinity interaction between bovine serum albumin and triazine dye in reversed micelles".APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY 95.3(2001):163-174.
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