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In vitro regeneration of loblolly pine and random amplified polymorphic DNA analyses of regenerated plantlets
Alternative TitlePlant Cell Reports
Tang, W
2001-02-01
Source PublicationPLANT CELL REPORTS
ISSN0721-7714
Volume20Issue:2Pages:163-168
AbstractAdventitious buds were induced from organogenic callus derived from mature zygotic embryos of three lines (E-311, E-440, and E-822) of loblolly pine (Pinus taeda L.) within 27 weeks of culture. The influence of cytokinins, silver nitrate, and low-temperature treatment on the differentiation of adventitious buds was analyzed. Elongation of adventitious buds was achieved on TE medium supplemented with 0.5 mg/l indole-3-butyric acid (IBA) and 1 mg/l 6-benzyladenine (BA). After adventitious shoots had rooted on TE medium supplemented with 0.5 mg/l IBA, 2 mg/l BA, and 0.5 mg/l gibberellic acid, 498 regenerated plantlets were transferred to a perlite:peatmoss:vermiculite (1:1:1) soil mixture; 351 of these survived in the field. Total DNA was extracted from 21 regenerated plantlets randomly chosen from the 151 regenerated plantlets of line E-822. Random amplified polymorphic DNA (RAPD) analysis using 80 arbitrary oligonucleotide 10-mers showed that 21 primers gave 107 clear reproducible bands, with the amplification products being monomorphic for all of the plantlets of line E-822 tested. A total of 2,247 bands obtained from these studies exhibited no aberration in RAPD banding patterns among the tested plantlets. These results suggest that somatic organogenesis can be used for clonal micropropagation of some lines of loblolly pine without the fear of the appearance of unwanted somaclonal variants.; Adventitious buds were induced from organogenic callus derived from mature zygotic embryos of three lines (E-311, E-440, and E-822) of loblolly pine (Pinus taeda L.) within 27 weeks of culture. The influence of cytokinins, silver nitrate, and low-temperature treatment on the differentiation of adventitious buds was analyzed. Elongation of adventitious buds was achieved on TE medium supplemented with 0.5 mg/l indole-3-butyric acid (IBA) and 1 mg/l 6-benzyladenine (BA). After adventitious shoots had rooted on TE medium supplemented with 0.5 mg/l IBA, 2 mg/l BA, and 0.5 mg/l gibberellic acid, 498 regenerated plantlets were transferred to a perlite:peatmoss:vermiculite (1:1:1) soil mixture; 351 of these survived in the field. Total DNA was extracted from 21 regenerated plantlets randomly chosen from the 151 regenerated plantlets of line E-822. Random amplified polymorphic DNA (RAPD) analysis using 80 arbitrary oligonucleotide 10-mers showed that 21 primers gave 107 clear reproducible bands, with the amplification products being monomorphic for all of the plantlets of line E-822 tested. A total of 2,247 bands obtained from these studies exhibited no aberration in RAPD banding patterns among the tested plantlets. These results suggest that somatic organogenesis can be used for clonal micropropagation of some lines of loblolly pine without the fear of the appearance of unwanted somaclonal variants.
KeywordPinus Taeda L. Organogenesis Plant Regeneration Random Amplified Polymorphic Dna
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
URL查看原文
Indexed BySCI
Language英语
WOS KeywordMATURE ZYGOTIC EMBRYOS ; RAPD MARKERS ; SOMATIC EMBRYOGENESIS ; SOMACLONAL VARIATION ; CALLUS-CULTURES ; TAEDA ; ORGANOGENESIS ; CONIFERS ; L.
WOS Research AreaPlant Sciences
WOS SubjectPlant Sciences
WOS IDWOS:000167189500010
Citation statistics
Cited Times:18[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Version出版稿
Identifierhttp://ir.ipe.ac.cn/handle/122111/5751
Collection研究所(批量导入)
Affiliation1.N Carolina State Univ, Dept Forestry, Forestry Biotechnol Grp, Raleigh, NC 27695 USA
2.Chinese Acad Sci, Inst Chem Met, State Key Lab Biochem Engn, Beijing 100080, Peoples R China
Recommended Citation
GB/T 7714
Tang, W. In vitro regeneration of loblolly pine and random amplified polymorphic DNA analyses of regenerated plantlets[J]. PLANT CELL REPORTS,2001,20(2):163-168.
APA Tang, W.(2001).In vitro regeneration of loblolly pine and random amplified polymorphic DNA analyses of regenerated plantlets.PLANT CELL REPORTS,20(2),163-168.
MLA Tang, W."In vitro regeneration of loblolly pine and random amplified polymorphic DNA analyses of regenerated plantlets".PLANT CELL REPORTS 20.2(2001):163-168.
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