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Optimization of conditions for bacteriocin extraction in PEG/salt aqueous two-phase systems using statistical experimental designs
Alternative TitleBiotechnol. Prog.
Li, C; Bai, JH; Li, W; Cai, ZL; Ouyang, F
2001-03-01
Source PublicationBIOTECHNOLOGY PROGRESS
ISSN8756-7938
Volume17Issue:2Pages:366-368
AbstractThe bacteriocin nisin was extracted in PEG/salt aqueous two-phase systems (ATPS) using the property that the systems can extract hydrophobic proteins. The concentrations of the phase-forming components, PEG 4000 and Na2SO4, were optimized for nisin recovery by means of statistical experimental designs, and it was found that they strongly influenced nisin recovery. The optimal composition of ATPS was found to be 15.99% (w/w) PEG 4000 and 15.85% (w/w) Na2SO4 (pH 2), and the optimal ATPS allowed an 11.60% increase of nisin recovery compared to the standard method of nisin assay.; The bacteriocin nisin was extracted in PEG/salt aqueous two-phase systems (ATPS) using the property that the systems can extract hydrophobic proteins. The concentrations of the phase-forming components, PEG 4000 and Na2SO4, were optimized for nisin recovery by means of statistical experimental designs, and it was found that they strongly influenced nisin recovery. The optimal composition of ATPS was found to be 15.99% (w/w) PEG 4000 and 15.85% (w/w) Na2SO4 (pH 2), and the optimal ATPS allowed an 11.60% increase of nisin recovery compared to the standard method of nisin assay.
KeywordLactic-acid Bacteria Response-surface Methodology Subsp Lactis Nisin Purification
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
URL查看原文
Indexed BySCI
Language英语
WOS KeywordLACTIC-ACID BACTERIA ; RESPONSE-SURFACE METHODOLOGY ; SUBSP LACTIS ; NISIN ; PURIFICATION
WOS Research AreaBiotechnology & Applied Microbiology ; Food Science & Technology
WOS SubjectBiotechnology & Applied Microbiology ; Food Science & Technology
WOS IDWOS:000168044700022
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Cited Times:43[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Version出版稿
Identifierhttp://ir.ipe.ac.cn/handle/122111/5772
Collection研究所(批量导入)
Affiliation1.Chinese Acad Sci, State Key Lab Biochem Engn, Inst Chem Met, Beijing 100080, Peoples R China
2.Hubei Univ, Sch Life Sci, Wuhan 430062, Peoples R China
Recommended Citation
GB/T 7714
Li, C,Bai, JH,Li, W,et al. Optimization of conditions for bacteriocin extraction in PEG/salt aqueous two-phase systems using statistical experimental designs[J]. BIOTECHNOLOGY PROGRESS,2001,17(2):366-368.
APA Li, C,Bai, JH,Li, W,Cai, ZL,&Ouyang, F.(2001).Optimization of conditions for bacteriocin extraction in PEG/salt aqueous two-phase systems using statistical experimental designs.BIOTECHNOLOGY PROGRESS,17(2),366-368.
MLA Li, C,et al."Optimization of conditions for bacteriocin extraction in PEG/salt aqueous two-phase systems using statistical experimental designs".BIOTECHNOLOGY PROGRESS 17.2(2001):366-368.
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