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Optimization of growth and jaceosidin production in callus and cell suspension cultures of Saussurea medusa
Alternative TitlePlant Cell Tissue Organ Cult.
Zhao, DX; Xing, JM; Li, MY; Lu, DP; Zhao, Q
2001-12-01
Source PublicationPLANT CELL TISSUE AND ORGAN CULTURE
ISSN0167-6857
Volume67Issue:3Pages:227-234
AbstractCalli cultures derived from the leaves of Saussurea medusa were selected on the basis of colour into three callus, A, B and C, which suggested different levels of metabolite accumulation. An improved reversed phase high performance liquid chromatographic method provided selective determination of the jaceosidin content of these samples. The jaceosidin concentration in callus B was higher than that of the callus A and C. By using 12-day old culture and 9-day old inoculum, jaceosidin yield of 72.91 mg l(-1)was obtained from cell line B in cell suspension cultures. The influence of some factors affecting jaceosidin formation, i.e. temperature, light, inoculum size, type of media, phytohormones, nitrogen and carbon source etc. were also examined. Light irradiation and combination of 3% (w/v) sucrose with 1% glucose brought about a marked increase of jaceosidin production. The effect of blue light on jaceosidin was markedly superior to other kinds of monochromatic light (red and far-red) or white light. Analysis of growth and jaceosidin content of callus cultures and cell suspension cultures demonstrated that the production of jaceosidin was growth-dependent in both cell solid culture and cell suspension culture.; Calli cultures derived from the leaves of Saussurea medusa were selected on the basis of colour into three callus, A, B and C, which suggested different levels of metabolite accumulation. An improved reversed phase high performance liquid chromatographic method provided selective determination of the jaceosidin content of these samples. The jaceosidin concentration in callus B was higher than that of the callus A and C. By using 12-day old culture and 9-day old inoculum, jaceosidin yield of 72.91 mg l(-1)was obtained from cell line B in cell suspension cultures. The influence of some factors affecting jaceosidin formation, i.e. temperature, light, inoculum size, type of media, phytohormones, nitrogen and carbon source etc. were also examined. Light irradiation and combination of 3% (w/v) sucrose with 1% glucose brought about a marked increase of jaceosidin production. The effect of blue light on jaceosidin was markedly superior to other kinds of monochromatic light (red and far-red) or white light. Analysis of growth and jaceosidin content of callus cultures and cell suspension cultures demonstrated that the production of jaceosidin was growth-dependent in both cell solid culture and cell suspension culture.
KeywordHigh Performance Liquid Chromatography Jaceosidin Plant Cell Culture Saussurea Medusa
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
URL查看原文
Indexed BySCI
Language英语
WOS KeywordFLAVONOIDS
WOS Research AreaBiotechnology & Applied Microbiology ; Plant Sciences
WOS SubjectBiotechnology & Applied Microbiology ; Plant Sciences
WOS IDWOS:000172074000003
Citation statistics
Cited Times:30[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Version出版稿
Identifierhttp://ir.ipe.ac.cn/handle/122111/5773
Collection研究所(批量导入)
Affiliation1.Chinese Acad Sci, Inst Bot, Beijing 100093, Peoples R China
2.Chinese Acad Sci, Inst Chem Met, Beijing 100080, Peoples R China
3.China Agr Univ, Beijing 100094, Peoples R China
Recommended Citation
GB/T 7714
Zhao, DX,Xing, JM,Li, MY,et al. Optimization of growth and jaceosidin production in callus and cell suspension cultures of Saussurea medusa[J]. PLANT CELL TISSUE AND ORGAN CULTURE,2001,67(3):227-234.
APA Zhao, DX,Xing, JM,Li, MY,Lu, DP,&Zhao, Q.(2001).Optimization of growth and jaceosidin production in callus and cell suspension cultures of Saussurea medusa.PLANT CELL TISSUE AND ORGAN CULTURE,67(3),227-234.
MLA Zhao, DX,et al."Optimization of growth and jaceosidin production in callus and cell suspension cultures of Saussurea medusa".PLANT CELL TISSUE AND ORGAN CULTURE 67.3(2001):227-234.
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