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Plant regeneration from embryogenic cultures initiated from mature loblolly pine zygotic embryos
Alternative TitleIn Vitro Cell. Dev. Biol.-Plant
Tang, W; Guo, ZC; Ouyang, F
2001-09-01
Source PublicationIN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT
ISSN1054-5476
Volume37Issue:5Pages:558-563
AbstractMature zygotic embryos of eight (open-pollinated) families of loblolly pine (Pinus taeda L.) were cultured on eight different basal salt formulations, each supplemented with 36.2 muM 2,4-dichlorophenoxyacetic acid, 17.8 muM 6-benzyladenine, 18.6 muM kinetin, 500 mg l(-1) casein hydrolysate, and 500 mg l(-1) L-glutamine for 9 wk; embryogenic tissue was formed on cotyledons, hypocotyls, and radicles of mature zygotic embryos. Callus was subcultured on the callus, proliferation, medium, the same as the induction medium, bat with one-fifth concentration of auxin and cytokinin for 9 wk. On this medium a white to translucent, glossy, mucilaginous embryogenic callus containing embryogenic suspensor masses (ESMs) was obtained. The highest frequency of explants forming embryogenic tissue, 17%, occurred on a modified Murashige and Skoog salts basal medium containing the concentration of KNO3, Ca(NO3)(2)-4H(2)O, NH4NO3, KCI, ZnSO4-7H(2)O(2), and MnSO4-H2O, 720, 1900, 00 250, 25.8, and 25.35 mg l(-1), respectively. Embryogenic suspension cultures were established by culturing embryogenic callus in liquid callus proliferation medium. Liquid cultures containing ESMs were transferred to medium containing abscisic acid, polyethylene glycols, and activated charcoal for stimulating ther production of cotyledonary somatic embryos. Mature somatic embryos germinated for 4-12 wk on medium containing indole-butyric acid, gibberellic acid, 6-benzyladenine, activated charcoal, and reduced sucrose concentration (15 g l(-1)). Two hundred and ninety-one regenerated plantlets were transferred to a perlite:peatmoss:vermiculite (1:1:1) mixture, then the plants were transplanted to soil in the earth, and 73 plantlets survived in the field.; Mature zygotic embryos of eight (open-pollinated) families of loblolly pine (Pinus taeda L.) were cultured on eight different basal salt formulations, each supplemented with 36.2 muM 2,4-dichlorophenoxyacetic acid, 17.8 muM 6-benzyladenine, 18.6 muM kinetin, 500 mg l(-1) casein hydrolysate, and 500 mg l(-1) L-glutamine for 9 wk; embryogenic tissue was formed on cotyledons, hypocotyls, and radicles of mature zygotic embryos. Callus was subcultured on the callus, proliferation, medium, the same as the induction medium, bat with one-fifth concentration of auxin and cytokinin for 9 wk. On this medium a white to translucent, glossy, mucilaginous embryogenic callus containing embryogenic suspensor masses (ESMs) was obtained. The highest frequency of explants forming embryogenic tissue, 17%, occurred on a modified Murashige and Skoog salts basal medium containing the concentration of KNO3, Ca(NO3)(2)-4H(2)O, NH4NO3, KCI, ZnSO4-7H(2)O(2), and MnSO4-H2O, 720, 1900, 00 250, 25.8, and 25.35 mg l(-1), respectively. Embryogenic suspension cultures were established by culturing embryogenic callus in liquid callus proliferation medium. Liquid cultures containing ESMs were transferred to medium containing abscisic acid, polyethylene glycols, and activated charcoal for stimulating ther production of cotyledonary somatic embryos. Mature somatic embryos germinated for 4-12 wk on medium containing indole-butyric acid, gibberellic acid, 6-benzyladenine, activated charcoal, and reduced sucrose concentration (15 g l(-1)). Two hundred and ninety-one regenerated plantlets were transferred to a perlite:peatmoss:vermiculite (1:1:1) mixture, then the plants were transplanted to soil in the earth, and 73 plantlets survived in the field.
KeywordPinus Taeda L. Embryogenic Cultures Somatic Embryogenesis Plant Regeneration
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
URL查看原文
Indexed BySCI
Language英语
WOS KeywordSOMATIC EMBRYOGENESIS ; PICEA-GLAUCA ; STORED SEEDS ; WHITE SPRUCE ; CALLUS ; POLYEMBRYOGENESIS ; INDUCTION ; CONIFERS ; MARIANA ; BLACK
WOS Research AreaPlant Sciences ; Cell Biology ; Developmental Biology
WOS SubjectPlant Sciences ; Cell Biology ; Developmental Biology
WOS IDWOS:000172369800009
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Cited Times:20[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Version出版稿
Identifierhttp://ir.ipe.ac.cn/handle/122111/5777
Collection研究所(批量导入)
Affiliation1.Chinese Acad Sci, Inst Bot, Lab Plant Cell Engn, Beijing 100093, Peoples R China
2.Chinese Acad Sci, Inst Chem Met, Biochem Engn Lab, Beijing 100080, Peoples R China
Recommended Citation
GB/T 7714
Tang, W,Guo, ZC,Ouyang, F. Plant regeneration from embryogenic cultures initiated from mature loblolly pine zygotic embryos[J]. IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT,2001,37(5):558-563.
APA Tang, W,Guo, ZC,&Ouyang, F.(2001).Plant regeneration from embryogenic cultures initiated from mature loblolly pine zygotic embryos.IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT,37(5),558-563.
MLA Tang, W,et al."Plant regeneration from embryogenic cultures initiated from mature loblolly pine zygotic embryos".IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT 37.5(2001):558-563.
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