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Studies of poly(vinyl acetate-co-diviyl benzene) beads as a carrier for the immobilization of penicillin acylase and the kinetics of immobilized penicillin acylase
Alternative TitleReact. Funct. Polym.
Liu, JG; Cong, W; Wang, S; Ouyang, F
2001-05-02
Source PublicationREACTIVE & FUNCTIONAL POLYMERS
ISSN1381-5148
Volume48Issue:1-3Pages:75-84
AbstractPoly(vinyl acetate-co-diviyl benzene) beads were synthesized via a novel polymerization method. The amount of hydroxide radical could reach 11.5 mmol g(-1) (wet) after the beads were catalyzed by NaOH in methanol at 18-20 degreesC for 24 h. The activators such as SESA, glutaraldehyde and p-benzoquinone were used to immobilize penicillin acylase. The penicillin acylase immobilized with SESA exhibited better stability than that with other activators. The successively immobilization could increase the activity of immobilized penicillin acylase about two times and the activity yield 10%, respectively, At the optimal conditions of immobilization, the activity of immobilized penicillin acylase amounted to 812-1200 unit per gram (wet). The kinetics of immobilized penicillin acylase was also studied. The reaction constants, K,, K-iPAA K-iAPA and K-s for immobilized penicillin acylase were equal to 0.033, 0.013, 0.009 and 0.081 mmol/ml, respectively. (C) 2001 Elsevier Science B.V. All rights reserved.; Poly(vinyl acetate-co-diviyl benzene) beads were synthesized via a novel polymerization method. The amount of hydroxide radical could reach 11.5 mmol g(-1) (wet) after the beads were catalyzed by NaOH in methanol at 18-20 degreesC for 24 h. The activators such as SESA, glutaraldehyde and p-benzoquinone were used to immobilize penicillin acylase. The penicillin acylase immobilized with SESA exhibited better stability than that with other activators. The successively immobilization could increase the activity of immobilized penicillin acylase about two times and the activity yield 10%, respectively, At the optimal conditions of immobilization, the activity of immobilized penicillin acylase amounted to 812-1200 unit per gram (wet). The kinetics of immobilized penicillin acylase was also studied. The reaction constants, K,, K-iPAA K-iAPA and K-s for immobilized penicillin acylase were equal to 0.033, 0.013, 0.009 and 0.081 mmol/ml, respectively. (C) 2001 Elsevier Science B.V. All rights reserved.
KeywordPoly(Vinyl Acetate-co-diviyl Benzene) Stability Immobilization Penicillin Acylase Kinetics
SubtypeArticle
WOS HeadingsScience & Technology ; Physical Sciences ; Technology
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Indexed ByISTP ; SCI
Language英语
WOS KeywordAFFINITY LIGANDS ; CHROMATOGRAPHY ; AGAROSE ; ENZYMES
WOS Research AreaChemistry ; Engineering ; Polymer Science
WOS SubjectChemistry, Applied ; Engineering, Chemical ; Polymer Science
WOS IDWOS:000169521800008
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Cited Times:9[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Version出版稿
Identifierhttp://ir.ipe.ac.cn/handle/122111/5792
Collection研究所(批量导入)
Affiliation1.Chinese Acad Sci, Inst Chem Met, Natl Lab Biochem Engn, Beijing 100080, Peoples R China
2.Shandong Univ, Dept Biol Sci, Shandong 270001, Peoples R China
Recommended Citation
GB/T 7714
Liu, JG,Cong, W,Wang, S,et al. Studies of poly(vinyl acetate-co-diviyl benzene) beads as a carrier for the immobilization of penicillin acylase and the kinetics of immobilized penicillin acylase[J]. REACTIVE & FUNCTIONAL POLYMERS,2001,48(1-3):75-84.
APA Liu, JG,Cong, W,Wang, S,&Ouyang, F.(2001).Studies of poly(vinyl acetate-co-diviyl benzene) beads as a carrier for the immobilization of penicillin acylase and the kinetics of immobilized penicillin acylase.REACTIVE & FUNCTIONAL POLYMERS,48(1-3),75-84.
MLA Liu, JG,et al."Studies of poly(vinyl acetate-co-diviyl benzene) beads as a carrier for the immobilization of penicillin acylase and the kinetics of immobilized penicillin acylase".REACTIVE & FUNCTIONAL POLYMERS 48.1-3(2001):75-84.
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