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芽孢杆菌固态发酵工艺研究
张怡
Subtype硕士
Thesis Advisor李佐虎
1991-06-30
Degree Grantor中国科学院研究生院
Abstract本文优化了苏云金杆固态发酵培养基成份及工艺条件,确立了控制发酵的关键因素,使Bt.vr.kurstaki平均发酵水平由160亿芽孢/g提高至220-250 亿芽孢/g,毒效7310U/mg。控制发酵的关键因素是适宜的碱性条件和Ca~(2+)浓度,适宜的碱性条件同时能控制发酵中霉菌的侵染;玉米粉是提高发酵产品毒力的关键因素之一,可使产品毒力有较大提高。紫外诱变筛选出培养性状较稳定的Bt9001菌株,发酵水平提高到270-330亿芽孢/g,毒效远高于Bt.var.kurstaki菌株。尝试了一级固态发酵工艺,从菌体生长过程阐述了一级与二级发酵工艺过程的区别,分析了产品毒效、发酵水平差别不大的原因。运用扩散原理,建立了Bt固态发酵生长数学模型,可恰当地描述不同发酵条件的生长适应期;对比了液态、固态发酵生长动力学的区别。dX/dt = μX(1-X/X_(max)) * 1/2(1-erf(1/2(uL/D)~(1/2)(1-t/τ)/(t/τ)~(1/2))) 对增产菌a47固态发酵工艺进行了初步实验研究,确定了麩皮和草碳为混合载体的固态发酵体系,使发酵水平提高到400-420亿芽孢/g。
Other AbstractIn this dissertation, the processes of Bacillus thuringiensis and Yield increased bacteria fermentation were investigated in solid state system, A variety of optimum culture conditions during Bt.var.kurstaki fer. mentation with respect to initial moisture content, temperature, seed amount and composition of solid medium were tested. The results were sat. isfactory with an average level of fermentation of 220-250 * 10~8 spores/g and the insecticidal activity of 7130IU/mg, which is quite higher than that of general submerged fermentation. A proper amount of alkali and Ca~(2+) was the key factors in the fermentation process. The mold contamination can be prevented successfully with proper amount of alkali in the medium. The effect of corn meal on the improvement of insecticidal activity was proved. Ultraviolet mutation breeding was used for seed screeming. Strain of Bt9001 obtained from this method had a high fermentation level of 270 - 330 * 10~8 spores/g, and its insecticidal activity was much higher than that of Bt.var.kurstaki. An equivalent result was obtained compared one-stage-inoculum fermentation with two-stage-inoculum fermentation and the reason was interpreted in detail from the point of view of growth kinetics. Besides, on the basis of the theory of dispersion, a mathematical model was established to describe Bacillus thuringiensis growth kinetics in solid state fermentation system, parameters of different runs were optimized. The model is: dX/dt = μX(1-X/X_(max)) * 1/2(1-erf(1/2(uL/D)~(1/2)(1-t/τ)/(t/τ)~(1/2))) A mixed-carrier system was chosen for the solid state fermentation of Yield increased bacteria a47, the level of fermentation could reach 400-420 * 10~8 spores/g.
Pages71
Language中文
Document Type学位论文
Identifierhttp://ir.ipe.ac.cn/handle/122111/5832
Collection研究所(批量导入)
Recommended Citation
GB/T 7714
张怡. 芽孢杆菌固态发酵工艺研究[D]. 中国科学院研究生院,1991.
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