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High-level succinic acid production and yield by lactose-induced expression of phosphoenolpyruvate carboxylase in ptsG mutant Escherichia coli
Wang, Dan1,2; Li, Qiang1,2; Mao, Yu3; Xing, Jianmin1; Su, Zhiguo1; Xing, JM
2010-08-01
Source PublicationAPPLIED MICROBIOLOGY AND BIOTECHNOLOGY
ISSN0175-7598
Volume87Issue:6Pages:2025-2035
AbstractEscherichia coli strains with foreign genes under the isopropyl-beta-d-thiogalactopyranoside-inducible promoters such as lac, tac, and trc were engineered and considered as the promising succinic acid-producing bacteria in many reports. The promoters mentioned above could also be induced by lactose, which had not been attempted for succinic acid production before. Here, the efficient utilization of lactose as inducer was demonstrated in cultures of the ptsG, ldhA, and pflB mutant strain DC1515 with ppc overexpression. A fermentative process for succinic acid production at high level by this strain was developed. In flask anaerobic culture, 14.86 g l(-1) succinic acid was produced from 15 g l(-1) glucose with a yield of 1.51 mol mol(-1) glucose. In two-stage culture carried out in a 3-l bioreactor, the overall yield and concentration of succinic acid reached to 1.67 mol mol(-1) glucose and 99.7 g l(-1), respectively, with a productivity of 1.7 g l(-1) h(-1) in the anaerobic stage. The efficient utilization of lactose as inducer made recombinant E. coli a more capable strain for succinic acid production at large scale.
KeywordGlucose Phosphotransferase (Ptsg) Escherichia Coli Succinic Acid Lactose Induction Phosphoenolpyruvate Carboxylase (Ppc)
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
DOI10.1007/s00253-010-2689-x
Indexed BySCI
Language英语
WOS KeywordRECOMBINANT PROTEIN-PRODUCTION ; FED-BATCH CULTURE ; PYRUVATE-CARBOXYLASE ; LAC PROMOTER ; STRAIN ; OVEREXPRESSION ; GENE ; FERMENTATION ; METABOLISM ; GLUCOSE
WOS Research AreaBiotechnology & Applied Microbiology
WOS SubjectBiotechnology & Applied Microbiology
WOS IDWOS:000280373100005
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Cited Times:19[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Version出版稿
Identifierhttp://ir.ipe.ac.cn/handle/122111/5835
Collection生化工程国家重点实验室
Corresponding AuthorXing, JM
Affiliation1.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China
2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
3.China Univ Min & Technol Beijing, Sch Chem & Environm Engn, Dept Bioengn, Beijing 100083, Peoples R China
Recommended Citation
GB/T 7714
Wang, Dan,Li, Qiang,Mao, Yu,et al. High-level succinic acid production and yield by lactose-induced expression of phosphoenolpyruvate carboxylase in ptsG mutant Escherichia coli[J]. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,2010,87(6):2025-2035.
APA Wang, Dan,Li, Qiang,Mao, Yu,Xing, Jianmin,Su, Zhiguo,&Xing, JM.(2010).High-level succinic acid production and yield by lactose-induced expression of phosphoenolpyruvate carboxylase in ptsG mutant Escherichia coli.APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,87(6),2025-2035.
MLA Wang, Dan,et al."High-level succinic acid production and yield by lactose-induced expression of phosphoenolpyruvate carboxylase in ptsG mutant Escherichia coli".APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 87.6(2010):2025-2035.
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