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Biochemical characterization of a maize stover beta-exoglucanase and its use in lignocellulose conversion
Han, Yejun; Chen, Hongzhang; Chen, HZ
2010-08-01
Source PublicationBIORESOURCE TECHNOLOGY
ISSN0960-8524
Volume101Issue:15Pages:6111-6117
AbstractPlant is one of the important resources for glycosyl hydrolase production. A beta-exoglucanase with molecular weight of 63.1 kDa was purified from fresh maize stover and subjected to enzymatic characterization. The optimal temperature and pH of the beta-exoglucanase was 40 degrees C and 6.0, respectively. The beta-exoglucanase was active against p-nitrophenyl-cellobiose (p-NPC), laminarin, cellotriose, cellotetraose, cellopentaose, Avicel, filter paper, and cotton cellulose. The analysis of hydrolytic mode suggested that the beta-exoglucanase removed cellobiose from the ends of beta-glucan. Kinetic parameters of the beta-exoglucanase for laminarin and p-NPC were determined. The effects of metal ions and chemical reagents on the beta-exoglucanase activity were also studied. The biochemical characterization of the beta-exoglucanase makes it an appealing cellulase additive in converting lignocelluloses to ethanol through simultaneous saccharification and fermentation. The synergism of the beta-exoglucanase or crude cell wall proteins of fresh maize stover with Trichoderma reesei cellulase was observed in ethanol production from lignocellulose. (C) 2010 Elsevier Ltd. All rights reserved.
KeywordBeta-exoglucanase Maize Stover Synergism Simultaneous Saccharification And Fermentation (Ssf)
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Technology
DOI10.1016/j.biortech.2010.02.108
Indexed BySCI
Language英语
WOS KeywordCOLEOPTILE CELL-WALLS ; SIMULTANEOUS SACCHARIFICATION ; MIXED LINKAGE ; CORN STOVER ; HYDROLASES ; ENDOGLUCANASES ; FERMENTATION ; PURIFICATION ; GLUCOSIDASE ; DEGRADATION
WOS Research AreaAgriculture ; Biotechnology & Applied Microbiology ; Energy & Fuels
WOS SubjectAgricultural Engineering ; Biotechnology & Applied Microbiology ; Energy & Fuels
WOS IDWOS:000278035900051
Citation statistics
Cited Times:7[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Version出版稿
Identifierhttp://ir.ipe.ac.cn/handle/122111/5837
Collection生化工程国家重点实验室
Corresponding AuthorChen, HZ
AffiliationChinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China
Recommended Citation
GB/T 7714
Han, Yejun,Chen, Hongzhang,Chen, HZ. Biochemical characterization of a maize stover beta-exoglucanase and its use in lignocellulose conversion[J]. BIORESOURCE TECHNOLOGY,2010,101(15):6111-6117.
APA Han, Yejun,Chen, Hongzhang,&Chen, HZ.(2010).Biochemical characterization of a maize stover beta-exoglucanase and its use in lignocellulose conversion.BIORESOURCE TECHNOLOGY,101(15),6111-6117.
MLA Han, Yejun,et al."Biochemical characterization of a maize stover beta-exoglucanase and its use in lignocellulose conversion".BIORESOURCE TECHNOLOGY 101.15(2010):6111-6117.
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