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Affinity extraction of BSA by mixed reversed micellar system with unbound triazine dye
Alternative TitleBiochem. Eng. J.
Zhang, TX; Liu, HZ; Chen, JY
1999-09-01
Source PublicationBIOCHEMICAL ENGINEERING JOURNAL
ISSN1369-703X
Volume4Issue:1Pages:17-21
AbstractThe affinity extraction of proteins with unbound triazine dye in the mixed reversed micellar system has been studied. Bovine serum albumin (BSA) and Cibacron Blue 3GA (CB) were chosen, respectively, as a model protein and an unbound ligand. The affinity CB in the aqueous phase was directly transferred to the mixed reversed micelles formed with cationic surfactant cetyltrimethylammonium bromide (CTAB) and tributyl phosphate (TBP). The transfer of BSA to the reversed micelles increases significantly by the biospecific interaction with the addition of a small amount of CB to the aqueous phase. For solutions with pH < pI, the selectivity of proteins can be expected with the presence of affinity CB since no BSA was found to be extracted into the reverse micelles without the addition of CB. The BSA transfer to the reversed micelles increases significantly by the addition of TBP to the organic phase. Most of BSA (similar to 82%) extracted into the reversed micelles can be back extracted into the aqueous phase by the increase of n-hexanol concentration in the organic phase while no CB would be transferred into the stripping aqueous solution at the same time. (C) 1999 Elsevier Science S.A. All rights reserved.; The affinity extraction of proteins with unbound triazine dye in the mixed reversed micellar system has been studied. Bovine serum albumin (BSA) and Cibacron Blue 3GA (CB) were chosen, respectively, as a model protein and an unbound ligand. The affinity CB in the aqueous phase was directly transferred to the mixed reversed micelles formed with cationic surfactant cetyltrimethylammonium bromide (CTAB) and tributyl phosphate (TBP). The transfer of BSA to the reversed micelles increases significantly by the biospecific interaction with the addition of a small amount of CB to the aqueous phase. For solutions with pH < pI, the selectivity of proteins can be expected with the presence of affinity CB since no BSA was found to be extracted into the reverse micelles without the addition of CB. The BSA transfer to the reversed micelles increases significantly by the addition of TBP to the organic phase. Most of BSA (similar to 82%) extracted into the reversed micelles can be back extracted into the aqueous phase by the increase of n-hexanol concentration in the organic phase while no CB would be transferred into the stripping aqueous solution at the same time. (C) 1999 Elsevier Science S.A. All rights reserved.
KeywordAffinity Extraction Reversed Micelles Cibacron Blue 3ga Bovine Serum Albumin
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Technology
URL查看原文
Indexed BySCI
Language英语
WOS KeywordPROTEIN EXTRACTION ; COSURFACTANT ; PURIFICATION ; SEPARATION ; ENZYMES
WOS Research AreaBiotechnology & Applied Microbiology ; Engineering
WOS SubjectBiotechnology & Applied Microbiology ; Engineering, Chemical
WOS IDWOS:000082915200003
Citation statistics
Cited Times:13[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Version出版稿
Identifierhttp://ir.ipe.ac.cn/handle/122111/5853
Collection研究所(批量导入)
AffiliationChinese Acad Sci, Young Scientist Lab Separat Sci & Engn, Inst Chem Met, Beijing 100080, Peoples R China
Recommended Citation
GB/T 7714
Zhang, TX,Liu, HZ,Chen, JY. Affinity extraction of BSA by mixed reversed micellar system with unbound triazine dye[J]. BIOCHEMICAL ENGINEERING JOURNAL,1999,4(1):17-21.
APA Zhang, TX,Liu, HZ,&Chen, JY.(1999).Affinity extraction of BSA by mixed reversed micellar system with unbound triazine dye.BIOCHEMICAL ENGINEERING JOURNAL,4(1),17-21.
MLA Zhang, TX,et al."Affinity extraction of BSA by mixed reversed micellar system with unbound triazine dye".BIOCHEMICAL ENGINEERING JOURNAL 4.1(1999):17-21.
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