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Affinity extraction of BSA with reversed micellar system composed of unbound Cibacron Blue
Alternative TitleBiotechnol. Prog.
Zhang, TX; Liu, HZ; Chen, JY
1999-11-01
Source PublicationBIOTECHNOLOGY PROGRESS
ISSN8756-7938
Volume15Issue:6Pages:1078-1082
AbstractAffinity Cibacron Blue 3GA (CB) dye in aqueous phase was directly transferred to the reversed micelles due to electrostatic interaction between anionic CB and cationic cetyltrimethylammonium bromide (CTAB). The bovine serum albumin (BSA) transfer to the reverse micelles increases significantly in a wide range of pH by the addition of a small amount of CB (similar to 1.0-7.0% of the total surfactant concentration) to the aqueous phase. For pH < pi, the selectivity can be significantly improved with the presence of affinity CB because no BSA was extracted in the absence of CB. For backward extraction of BSA from the micellar phase with stripping aqueous solution, the addition of 2-propanol to the aqueous phase can recover almost all BSA (98.5%) extracted into the reverse micelles.; Affinity Cibacron Blue 3GA (CB) dye in aqueous phase was directly transferred to the reversed micelles due to electrostatic interaction between anionic CB and cationic cetyltrimethylammonium bromide (CTAB). The bovine serum albumin (BSA) transfer to the reverse micelles increases significantly in a wide range of pH by the addition of a small amount of CB (similar to 1.0-7.0% of the total surfactant concentration) to the aqueous phase. For pH < pi, the selectivity can be significantly improved with the presence of affinity CB because no BSA was extracted in the absence of CB. For backward extraction of BSA from the micellar phase with stripping aqueous solution, the addition of 2-propanol to the aqueous phase can recover almost all BSA (98.5%) extracted into the reverse micelles.
KeywordLiquid-liquid-extraction Protein Extraction Separations Surfactant Enzymes
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
URL查看原文
Indexed BySCI
Language英语
WOS KeywordLIQUID-LIQUID-EXTRACTION ; PROTEIN EXTRACTION ; SEPARATIONS ; SURFACTANT ; ENZYMES
WOS Research AreaBiotechnology & Applied Microbiology ; Food Science & Technology
WOS SubjectBiotechnology & Applied Microbiology ; Food Science & Technology
WOS IDWOS:000084121500016
Citation statistics
Cited Times:14[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Version出版稿
Identifierhttp://ir.ipe.ac.cn/handle/122111/5854
Collection研究所(批量导入)
AffiliationChinese Acad Sci, Inst Chem Met, Lab Separat Sci & Engn, Beijing 100080, Peoples R China
Recommended Citation
GB/T 7714
Zhang, TX,Liu, HZ,Chen, JY. Affinity extraction of BSA with reversed micellar system composed of unbound Cibacron Blue[J]. BIOTECHNOLOGY PROGRESS,1999,15(6):1078-1082.
APA Zhang, TX,Liu, HZ,&Chen, JY.(1999).Affinity extraction of BSA with reversed micellar system composed of unbound Cibacron Blue.BIOTECHNOLOGY PROGRESS,15(6),1078-1082.
MLA Zhang, TX,et al."Affinity extraction of BSA with reversed micellar system composed of unbound Cibacron Blue".BIOTECHNOLOGY PROGRESS 15.6(1999):1078-1082.
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