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Affinity-based reversed micellar bovine serum albumin (BSA) extraction with unbound reactive dye
Alternative TitleSep. Sci. Technol.
Zhang, TX; Liu, HZ; Chen, JY
2000
Source PublicationSEPARATION SCIENCE AND TECHNOLOGY
ISSN0149-6395
Volume35Issue:1Pages:143-151
AbstractA quick and simple method has been introduced for affinity extraction with an unbound reactive dye in a reversed micellar system. Bovine serum albumin (BSA) and Cibacron Blue 3GA (CB) were chosen, respectively, as a model protein and an unbound ligand. CB in the aqueous phase was directly transferred to the reversed micelles due to electrostatic interaction between anionic CB and cationic cetyltrimethylammonium bromide. The BSA transfer to the reversed micelles increases significantly by affinity interaction with the addition of a small amount of CB to the aqueous phase. For solutions with pH close to pI, the selectivity of proteins can be expected in the presence of affinity CB as very little BSA is extracted into the reverse micelles without the addition of CB. Most of the BSA (similar to 82%) extracted into the reversed micelles can be backextracted into the stripping aqueous phase by increasing of n-hexanol concentration in the organic phase while no CB is transferred to the aqueous solution at the same time.; A quick and simple method has been introduced for affinity extraction with an unbound reactive dye in a reversed micellar system. Bovine serum albumin (BSA) and Cibacron Blue 3GA (CB) were chosen, respectively, as a model protein and an unbound ligand. CB in the aqueous phase was directly transferred to the reversed micelles due to electrostatic interaction between anionic CB and cationic cetyltrimethylammonium bromide. The BSA transfer to the reversed micelles increases significantly by affinity interaction with the addition of a small amount of CB to the aqueous phase. For solutions with pH close to pI, the selectivity of proteins can be expected in the presence of affinity CB as very little BSA is extracted into the reverse micelles without the addition of CB. Most of the BSA (similar to 82%) extracted into the reversed micelles can be backextracted into the stripping aqueous phase by increasing of n-hexanol concentration in the organic phase while no CB is transferred to the aqueous solution at the same time.
KeywordAffinity Extraction Reversed Micelles Cibacron Blue 3ga Bovine Serum Albumin
SubtypeArticle
WOS HeadingsScience & Technology ; Physical Sciences ; Technology
URL查看原文
Indexed BySCI
Language英语
WOS KeywordPROTEIN EXTRACTION ; NONIONIC SURFACTANT ; BIOAFFINITY ; SEPARATION ; CHROMATOGRAPHY ; COSURFACTANT ; PURIFICATION ; PERFORMANCE ; TRYPSIN ; SYSTEMS
WOS Research AreaChemistry ; Engineering
WOS SubjectChemistry, Multidisciplinary ; Engineering, Chemical
WOS IDWOS:000084758900009
Citation statistics
Cited Times:8[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Version出版稿
Identifierhttp://ir.ipe.ac.cn/handle/122111/5855
Collection研究所(批量导入)
AffiliationChinese Acad Sci, Inst Chem Met, Young Scientist Lab Separat Sci & Engn, Beijing 100080, Peoples R China
Recommended Citation
GB/T 7714
Zhang, TX,Liu, HZ,Chen, JY. Affinity-based reversed micellar bovine serum albumin (BSA) extraction with unbound reactive dye[J]. SEPARATION SCIENCE AND TECHNOLOGY,2000,35(1):143-151.
APA Zhang, TX,Liu, HZ,&Chen, JY.(2000).Affinity-based reversed micellar bovine serum albumin (BSA) extraction with unbound reactive dye.SEPARATION SCIENCE AND TECHNOLOGY,35(1),143-151.
MLA Zhang, TX,et al."Affinity-based reversed micellar bovine serum albumin (BSA) extraction with unbound reactive dye".SEPARATION SCIENCE AND TECHNOLOGY 35.1(2000):143-151.
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