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Application of microwave energy in sample preparation for analysis of trehalose in yeast
Alternative TitleChin. J. Anal. Chem.
Liu, CB; Li, N; Lu, JQ; Miao, WR; Su, ZG
1999
Source PublicationCHINESE JOURNAL OF ANALYTICAL CHEMISTRY
ISSN0253-3820
Volume27Issue:1Pages:24-28
AbstractA sample preparation method was established for extracting trehalose from yeast with the aid of microwave energy. The high performance liquid chromatographic separation and determination of the extracted compounds showed that the microwave extraction method was more advantageous than traditional extraction method with regard to the selectivity of extraction and the time and cost of the procedure. As trehalase was inactivated after 20 s microwave irradiation, the change of trehalose degradation in extracting process may be considerably reduced. Microwave extraction seems to be an effective sample preparation method for trehalose determination expecially in the study of yeast metabolism.; A sample preparation method was established for extracting trehalose from yeast with the aid of microwave energy. The high performance liquid chromatographic separation and determination of the extracted compounds showed that the microwave extraction method was more advantageous than traditional extraction method with regard to the selectivity of extraction and the time and cost of the procedure. As trehalase was inactivated after 20 s microwave irradiation, the change of trehalose degradation in extracting process may be considerably reduced. Microwave extraction seems to be an effective sample preparation method for trehalose determination expecially in the study of yeast metabolism.
KeywordMicrowave High Performance Liquid Chromatography Sample Preparation Trehalose
SubtypeArticle
WOS HeadingsScience & Technology ; Physical Sciences
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Indexed BySCI
Language英语
WOS KeywordSACCHAROMYCES-CEREVISIAE ; METABOLISM
WOS Research AreaChemistry
WOS SubjectChemistry, Analytical
WOS IDWOS:000080031800006
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Document Type期刊论文
Version出版稿
Identifierhttp://ir.ipe.ac.cn/handle/122111/5860
Collection研究所(批量导入)
Affiliation1.Dalian Univ Technol, Inst Biochem Engn, Dalian 116012, Peoples R China
2.Dalian Univ Technol, State Key Lab Fine Chem, Dalian 116012, Peoples R China
3.Chinese Acad Sci, Inst Chem Met, State Key Lab Biochem Engn, Beijing 100080, Peoples R China
Recommended Citation
GB/T 7714
Liu, CB,Li, N,Lu, JQ,et al. Application of microwave energy in sample preparation for analysis of trehalose in yeast[J]. CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,1999,27(1):24-28.
APA Liu, CB,Li, N,Lu, JQ,Miao, WR,&Su, ZG.(1999).Application of microwave energy in sample preparation for analysis of trehalose in yeast.CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,27(1),24-28.
MLA Liu, CB,et al."Application of microwave energy in sample preparation for analysis of trehalose in yeast".CHINESE JOURNAL OF ANALYTICAL CHEMISTRY 27.1(1999):24-28.
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微波能用于酵母中海藻糖高效液相色谱分析样(340KB) 限制开放CC BY-NC-SAApplication Full Text
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