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Artemisinin production by adventitious shoots of Artemisia annua in a novel mist bioreactor
Alternative TitleActa Bot. Sin.
Lii, CZ; Wang, YC; Kang, XZ; Fan, OY; Ye, HC; Li, GF
1999-05-01
Source PublicationACTA BOTANICA SINICA
ISSN0577-7496
Volume41Issue:5Pages:524-527
AbstractA novel ultrasonic inner-loop bioreactor was used for artemisinin production by adventitious shoots in a multiplate culture of Artemisia annua L. The bioreactor was designed to allow the nutrient mist to uprise along a concentric draught-cylinder until it overflows from the top opening and the side-holes of the central tube downward and out of the annulus, so that the nutrient mist can be fulfilled in the bioreactor within 2 similar to 3 minutes. Under the misting cycles of every 3-minute misting in every 90 minute interval, artemisinin production reached totally 46.9 mg DW/L of culture medium at an airflow rate of 0.5 L/min for 25 d of culture in batches. The product amounted 2.9 and 3.2 folds of those obtained from culturing in solid medium and in shaking flasks respectively.; A novel ultrasonic inner-loop bioreactor was used for artemisinin production by adventitious shoots in a multiplate culture of Artemisia annua L. The bioreactor was designed to allow the nutrient mist to uprise along a concentric draught-cylinder until it overflows from the top opening and the side-holes of the central tube downward and out of the annulus, so that the nutrient mist can be fulfilled in the bioreactor within 2 similar to 3 minutes. Under the misting cycles of every 3-minute misting in every 90 minute interval, artemisinin production reached totally 46.9 mg DW/L of culture medium at an airflow rate of 0.5 L/min for 25 d of culture in batches. The product amounted 2.9 and 3.2 folds of those obtained from culturing in solid medium and in shaking flasks respectively.
KeywordArtemisia Annua Mist Bioreactor Adventitious Shoot Artemisinin
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
URL查看原文
Indexed BySCI
Language英语
WOS KeywordQINGHAOSU ARTEMISININ ; ANTIMALARIAL DRUG ; CULTURES
WOS Research AreaBiochemistry & Molecular Biology ; Plant Sciences
WOS SubjectBiochemistry & Molecular Biology ; Plant Sciences
WOS IDWOS:000082558200016
Citation statistics
Document Type期刊论文
Version出版稿
Identifierhttp://ir.ipe.ac.cn/handle/122111/5861
Collection研究所(批量导入)
Affiliation1.Chinese Acad Sci, Inst Chem Met, State Key Lab Biochem Engn, Beijing 100080, Peoples R China
2.Chinese Acad Sci, Inst Bot, Beijing 100093, Peoples R China
Recommended Citation
GB/T 7714
Lii, CZ,Wang, YC,Kang, XZ,et al. Artemisinin production by adventitious shoots of Artemisia annua in a novel mist bioreactor[J]. ACTA BOTANICA SINICA,1999,41(5):524-527.
APA Lii, CZ,Wang, YC,Kang, XZ,Fan, OY,Ye, HC,&Li, GF.(1999).Artemisinin production by adventitious shoots of Artemisia annua in a novel mist bioreactor.ACTA BOTANICA SINICA,41(5),524-527.
MLA Lii, CZ,et al."Artemisinin production by adventitious shoots of Artemisia annua in a novel mist bioreactor".ACTA BOTANICA SINICA 41.5(1999):524-527.
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