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Cell growth and flavonoids production in suspension culture of Saussurea medusa
Alternative TitleActa Bot. Sin.
Xing, JM; Zhao, DX; Li, MY; Ye, HC; Li, GF; Li, ZH
1998
Source PublicationACTA BOTANICA SINICA
ISSN0577-7496
Volume40Issue:9Pages:836-841
AbstractCell suspension cultures were established from Saussurea medusa Maxim, callus cultures. The effects of different rotation speeds of the gyratory shaker, different inoculum sizes and different pH values of the medium on cell growth and flavonoid formation were studied. The result showed that the optimum rotation, speed, inoculum size and initial pH value of the medium were 90 similar to 120 r/min, 50 similar to 80 g FW/L and 5.5 similar to 6.0 respectively for cell growth and flavonoids formation in the suspension cultures. Sucrose was better than glucose and fructose for the suspension cultures. The optimum concentration of sucrose for cell growth and flavonoid production was 40 g/L, and the concentration of flavonoids could be as high as 1 423.25 mg/L. High performance liquid chromatographic analysis of cell suspension culture extracts showed that the concentrations of jaceosidin and hispidulin in the flavonoids were 22.11% and 0.15% respectively.; Cell suspension cultures were established from Saussurea medusa Maxim, callus cultures. The effects of different rotation speeds of the gyratory shaker, different inoculum sizes and different pH values of the medium on cell growth and flavonoid formation were studied. The result showed that the optimum rotation, speed, inoculum size and initial pH value of the medium were 90 similar to 120 r/min, 50 similar to 80 g FW/L and 5.5 similar to 6.0 respectively for cell growth and flavonoids formation in the suspension cultures. Sucrose was better than glucose and fructose for the suspension cultures. The optimum concentration of sucrose for cell growth and flavonoid production was 40 g/L, and the concentration of flavonoids could be as high as 1 423.25 mg/L. High performance liquid chromatographic analysis of cell suspension culture extracts showed that the concentrations of jaceosidin and hispidulin in the flavonoids were 22.11% and 0.15% respectively.
KeywordSaussurea Medusa Suspension Culture Cell Growth Flavonoids Production
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
URL查看原文
Indexed BySCI
Language英语
WOS Research AreaBiochemistry & Molecular Biology ; Plant Sciences
WOS SubjectBiochemistry & Molecular Biology ; Plant Sciences
WOS IDWOS:000076577300011
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Cited Times:6[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Version出版稿
Identifierhttp://ir.ipe.ac.cn/handle/122111/5870
Collection研究所(批量导入)
Affiliation1.Chinese Acad Sci, Inst Bot, Beijing 100093, Peoples R China
2.Chinese Acad Sci, Inst Chem Met, Beijing 100080, Peoples R China
Recommended Citation
GB/T 7714
Xing, JM,Zhao, DX,Li, MY,et al. Cell growth and flavonoids production in suspension culture of Saussurea medusa[J]. ACTA BOTANICA SINICA,1998,40(9):836-841.
APA Xing, JM,Zhao, DX,Li, MY,Ye, HC,Li, GF,&Li, ZH.(1998).Cell growth and flavonoids production in suspension culture of Saussurea medusa.ACTA BOTANICA SINICA,40(9),836-841.
MLA Xing, JM,et al."Cell growth and flavonoids production in suspension culture of Saussurea medusa".ACTA BOTANICA SINICA 40.9(1998):836-841.
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水母雪莲悬浮培养细胞生长和黄酮类活性成分(203KB) 限制开放CC BY-NC-SAApplication Full Text
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