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Development of a nutrient mist bioreactor for growth of hairy roots
Alternative TitleIn Vitro Cell. Dev. Biol.-Plant
Liu, CZ; Wang, YC; Zhao, B; Guo, C; Ouyang, F; Ye, HC; Li, GF
1999-05-01
Source PublicationIN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT
ISSN1054-5476
Volume35Issue:3Pages:271-274
AbstractHairy root cultures of Artemisia annua L. were cultivated in three different mist bioreactors, each fitted with three stainless steel meshes. The growth rates in the three 2.3-L mist bioreactors differed. After 25 d, the growth index (final dry weight/initial dry weight) of the roots was 42 in a nutrient mist bioreactor, 61 in an inner-loop nutrient mist bioreactor; and 68 in a modified inner-loop nutrient mist bioreactor. Under a misting cycle of 3/30 (ON 3 min/OFF 30 min) for 25 d, dry weight reached 13.6 g/L of medium in the modified inner-loop nutrient mist bioreactor in which nutrient could be supplied without dilution of mist tv air flow.; Hairy root cultures of Artemisia annua L. were cultivated in three different mist bioreactors, each fitted with three stainless steel meshes. The growth rates in the three 2.3-L mist bioreactors differed. After 25 d, the growth index (final dry weight/initial dry weight) of the roots was 42 in a nutrient mist bioreactor, 61 in an inner-loop nutrient mist bioreactor; and 68 in a modified inner-loop nutrient mist bioreactor. Under a misting cycle of 3/30 (ON 3 min/OFF 30 min) for 25 d, dry weight reached 13.6 g/L of medium in the modified inner-loop nutrient mist bioreactor in which nutrient could be supplied without dilution of mist tv air flow.
KeywordArtemisia Annua L. Growth Hairy Root Cultures Mist Bioreactor
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
URL查看原文
Indexed BySCI
Language英语
WOS KeywordARTEMISIA-ANNUA L ; SHOOT CULTURES ; REACTOR ; SYSTEM
WOS Research AreaPlant Sciences ; Cell Biology ; Developmental Biology
WOS SubjectPlant Sciences ; Cell Biology ; Developmental Biology
WOS IDWOS:000081093400017
Citation statistics
Cited Times:28[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Version出版稿
Identifierhttp://ir.ipe.ac.cn/handle/122111/5887
Collection研究所(批量导入)
Affiliation1.Chinese Acad Sci, State Key Lab Biochem Engn, Inst Chem Met, Beijing 100080, Peoples R China
2.Chinese Acad Sci, Inst Bot, Beijing 100093, Peoples R China
Recommended Citation
GB/T 7714
Liu, CZ,Wang, YC,Zhao, B,et al. Development of a nutrient mist bioreactor for growth of hairy roots[J]. IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT,1999,35(3):271-274.
APA Liu, CZ.,Wang, YC.,Zhao, B.,Guo, C.,Ouyang, F.,...&Li, GF.(1999).Development of a nutrient mist bioreactor for growth of hairy roots.IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT,35(3),271-274.
MLA Liu, CZ,et al."Development of a nutrient mist bioreactor for growth of hairy roots".IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT 35.3(1999):271-274.
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