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Effect of the cosolvent type on the extraction of alpha-amylase with reversed micelles: Circular dichroism study
Alternative TitleEnzyme Microb. Technol.
Chang, Q. L.; Chen, J. Y.; Zhang, X. F.; Zhao, N. M.
1997
Source PublicationEnzyme and Microbial Technology
Issue2Pages:87-92
AbstractBoth the activity and conformational properties of alpha-amylase during the reversed micellar liquid-liquid extraction were investigated by the measurement of enzyme activity and circular dichroism (CD) probe analysis, respectively. The measurement of alpha-amylase activity indicated that only n-butanol, among the six n-alcohols (n-butanol, n-pentanol, n-hexanol, n-heptanol, n-octanol, and n-decanol), was the most suitable cosolvent for Aliquat 336 reversed micelles to extract and recover alpha-amylase in a full reversed micellar extraction cycle. In this case, as high as 80% of the total activity of alpha-amylase in the initial aqueous phase could be recovered after the forward and backward extraction cycle. For the other five n-alcohols, however, the recovery of enzyme activity was relatively low. Meanwhile, the CD spectra analysis during the same extraction processes revealed some similar behaviors on the conformational change of alpha-amylase. In the case of n-butanol as the cosolvent, it was found that the conformation of alpha-amylase in the stripping solution could be restored almost completely to its native state after. an extraction cycle; this implied that most of the enzyme activity would be recovered under this condition. For the other five cosolvents, it was found that the conformation of alpha-amylase could be restored partly to its native state after the full extraction cycle which demonstrated the fact obtained from the activity measurement experiments that only small parts of the total activity of alpha-amylase in the initial aqueous solution could be recovered at the end of an extraction cycle. Both the measurement of enzyme activity and CD spectra analysis proved that n-butanol was the most suitable cosolvent for Aliquat 336 reversed micelles to extract alpha-amylase. This observation may be helpful in developing a more effective method to monitor the process of protein reversed micellar extraction. (C) 1997 by Elsevier Science Inc.
KeywordCircular Dichroism Reversed Micelles Extraction Alpha-amylase Cosolvent Activity Liquid-liquid-extraction Purification Separation Recovery Protein
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Indexed BySCI
Language英语
Document Type期刊论文
Version出版稿
Identifierhttp://ir.ipe.ac.cn/handle/122111/5900
Collection研究所(批量导入)
Recommended Citation
GB/T 7714
Chang, Q. L.,Chen, J. Y.,Zhang, X. F.,et al. Effect of the cosolvent type on the extraction of alpha-amylase with reversed micelles: Circular dichroism study[J]. Enzyme and Microbial Technology,1997(2):87-92.
APA Chang, Q. L.,Chen, J. Y.,Zhang, X. F.,&Zhao, N. M..(1997).Effect of the cosolvent type on the extraction of alpha-amylase with reversed micelles: Circular dichroism study.Enzyme and Microbial Technology(2),87-92.
MLA Chang, Q. L.,et al."Effect of the cosolvent type on the extraction of alpha-amylase with reversed micelles: Circular dichroism study".Enzyme and Microbial Technology .2(1997):87-92.
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