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EFFECT OF THE TYPE OF COSOLVENT ON THE EXTRACTION PROCESS FOR SEPARATION AND PURIFICATION OF 2 ENZYMES FROM BACILLUS-SUBTILIS USING ALIQUAT-336 REVERSED MICELLES | |
Alternative Title | Sep. Sci. Technol. |
CHANG, QL; CHEN, JY | |
1995 | |
Source Publication | SEPARATION SCIENCE AND TECHNOLOGY
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ISSN | 0149-6395 |
Volume | 30Issue:13Pages:2679-2693 |
Abstract | Seven kinds of alkyl alcohol (n-butanol, n-pentanol, n-hexanol, n-heptanol, n-octanol, n-nonanol, and n-decanol) were chosen as the cosolvent for Aliquat 336/ isooctane reversed micelles. By performing liquid-liquid extraction with Aliquat 336 reversed micelles as the extractant, the separation and purification of two enzymes (alpha-amylase and neutral protease) from Bacillus subtilis were investigated. Experiments revealed that when n-butanol is used as the cosolvent, the two enzymes from Bacillus subtilis can be effectively separated and purified after a full forward and backward extraction cycle. alpha-Amylase was separated in the stripping solution with about 85% of its total activity recovered and purified about 1.6-fold. Neutral protease was separated in the raffinate with about 80% of its total activity recovered and purified about 3.5-fold. For the other six alcohols used as the cosolvent for Aliquat 336/isooctane reversed micelles, separation was not achieved.; Seven kinds of alkyl alcohol (n-butanol, n-pentanol, n-hexanol, n-heptanol, n-octanol, n-nonanol, and n-decanol) were chosen as the cosolvent for Aliquat 336/ isooctane reversed micelles. By performing liquid-liquid extraction with Aliquat 336 reversed micelles as the extractant, the separation and purification of two enzymes (alpha-amylase and neutral protease) from Bacillus subtilis were investigated. Experiments revealed that when n-butanol is used as the cosolvent, the two enzymes from Bacillus subtilis can be effectively separated and purified after a full forward and backward extraction cycle. alpha-Amylase was separated in the stripping solution with about 85% of its total activity recovered and purified about 1.6-fold. Neutral protease was separated in the raffinate with about 80% of its total activity recovered and purified about 3.5-fold. For the other six alcohols used as the cosolvent for Aliquat 336/isooctane reversed micelles, separation was not achieved. |
Keyword | Reversed Micelles Cosolvent Separation Alpha-amylase Neutral Protease |
Subtype | Article |
WOS Headings | Science & Technology ; Physical Sciences ; Technology |
URL | 查看原文 |
Indexed By | SCI |
Language | 英语 |
WOS Keyword | LIQUID-LIQUID-EXTRACTION ; ALPHA-CHYMOTRYPSIN ; SOLUBILIZATION ; RECOVERY |
WOS Research Area | Chemistry ; Engineering |
WOS Subject | Chemistry, Multidisciplinary ; Engineering, Chemical |
WOS ID | WOS:A1995RL69100004 |
Citation statistics | |
Document Type | 期刊论文 |
Version | 出版稿 |
Identifier | http://ir.ipe.ac.cn/handle/122111/5901 |
Collection | 研究所(批量导入) |
Recommended Citation GB/T 7714 | CHANG, QL,CHEN, JY. EFFECT OF THE TYPE OF COSOLVENT ON THE EXTRACTION PROCESS FOR SEPARATION AND PURIFICATION OF 2 ENZYMES FROM BACILLUS-SUBTILIS USING ALIQUAT-336 REVERSED MICELLES[J]. SEPARATION SCIENCE AND TECHNOLOGY,1995,30(13):2679-2693. |
APA | CHANG, QL,&CHEN, JY.(1995).EFFECT OF THE TYPE OF COSOLVENT ON THE EXTRACTION PROCESS FOR SEPARATION AND PURIFICATION OF 2 ENZYMES FROM BACILLUS-SUBTILIS USING ALIQUAT-336 REVERSED MICELLES.SEPARATION SCIENCE AND TECHNOLOGY,30(13),2679-2693. |
MLA | CHANG, QL,et al."EFFECT OF THE TYPE OF COSOLVENT ON THE EXTRACTION PROCESS FOR SEPARATION AND PURIFICATION OF 2 ENZYMES FROM BACILLUS-SUBTILIS USING ALIQUAT-336 REVERSED MICELLES".SEPARATION SCIENCE AND TECHNOLOGY 30.13(1995):2679-2693. |
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