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EFFECT OF THE TYPE OF COSOLVENT ON THE EXTRACTION PROCESS FOR SEPARATION AND PURIFICATION OF 2 ENZYMES FROM BACILLUS-SUBTILIS USING ALIQUAT-336 REVERSED MICELLES
Alternative TitleSep. Sci. Technol.
CHANG, QL; CHEN, JY
1995
Source PublicationSEPARATION SCIENCE AND TECHNOLOGY
ISSN0149-6395
Volume30Issue:13Pages:2679-2693
AbstractSeven kinds of alkyl alcohol (n-butanol, n-pentanol, n-hexanol, n-heptanol, n-octanol, n-nonanol, and n-decanol) were chosen as the cosolvent for Aliquat 336/ isooctane reversed micelles. By performing liquid-liquid extraction with Aliquat 336 reversed micelles as the extractant, the separation and purification of two enzymes (alpha-amylase and neutral protease) from Bacillus subtilis were investigated. Experiments revealed that when n-butanol is used as the cosolvent, the two enzymes from Bacillus subtilis can be effectively separated and purified after a full forward and backward extraction cycle. alpha-Amylase was separated in the stripping solution with about 85% of its total activity recovered and purified about 1.6-fold. Neutral protease was separated in the raffinate with about 80% of its total activity recovered and purified about 3.5-fold. For the other six alcohols used as the cosolvent for Aliquat 336/isooctane reversed micelles, separation was not achieved.; Seven kinds of alkyl alcohol (n-butanol, n-pentanol, n-hexanol, n-heptanol, n-octanol, n-nonanol, and n-decanol) were chosen as the cosolvent for Aliquat 336/ isooctane reversed micelles. By performing liquid-liquid extraction with Aliquat 336 reversed micelles as the extractant, the separation and purification of two enzymes (alpha-amylase and neutral protease) from Bacillus subtilis were investigated. Experiments revealed that when n-butanol is used as the cosolvent, the two enzymes from Bacillus subtilis can be effectively separated and purified after a full forward and backward extraction cycle. alpha-Amylase was separated in the stripping solution with about 85% of its total activity recovered and purified about 1.6-fold. Neutral protease was separated in the raffinate with about 80% of its total activity recovered and purified about 3.5-fold. For the other six alcohols used as the cosolvent for Aliquat 336/isooctane reversed micelles, separation was not achieved.
KeywordReversed Micelles Cosolvent Separation Alpha-amylase Neutral Protease
SubtypeArticle
WOS HeadingsScience & Technology ; Physical Sciences ; Technology
URL查看原文
Indexed BySCI
Language英语
WOS KeywordLIQUID-LIQUID-EXTRACTION ; ALPHA-CHYMOTRYPSIN ; SOLUBILIZATION ; RECOVERY
WOS Research AreaChemistry ; Engineering
WOS SubjectChemistry, Multidisciplinary ; Engineering, Chemical
WOS IDWOS:A1995RL69100004
Citation statistics
Cited Times:3[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Version出版稿
Identifierhttp://ir.ipe.ac.cn/handle/122111/5901
Collection研究所(批量导入)
Recommended Citation
GB/T 7714
CHANG, QL,CHEN, JY. EFFECT OF THE TYPE OF COSOLVENT ON THE EXTRACTION PROCESS FOR SEPARATION AND PURIFICATION OF 2 ENZYMES FROM BACILLUS-SUBTILIS USING ALIQUAT-336 REVERSED MICELLES[J]. SEPARATION SCIENCE AND TECHNOLOGY,1995,30(13):2679-2693.
APA CHANG, QL,&CHEN, JY.(1995).EFFECT OF THE TYPE OF COSOLVENT ON THE EXTRACTION PROCESS FOR SEPARATION AND PURIFICATION OF 2 ENZYMES FROM BACILLUS-SUBTILIS USING ALIQUAT-336 REVERSED MICELLES.SEPARATION SCIENCE AND TECHNOLOGY,30(13),2679-2693.
MLA CHANG, QL,et al."EFFECT OF THE TYPE OF COSOLVENT ON THE EXTRACTION PROCESS FOR SEPARATION AND PURIFICATION OF 2 ENZYMES FROM BACILLUS-SUBTILIS USING ALIQUAT-336 REVERSED MICELLES".SEPARATION SCIENCE AND TECHNOLOGY 30.13(1995):2679-2693.
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