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| EFFECT OF THE TYPE OF COSOLVENT ON THE EXTRACTION PROCESS FOR SEPARATION AND PURIFICATION OF 2 ENZYMES FROM BACILLUS-SUBTILIS USING ALIQUAT-336 REVERSED MICELLES | |
| Alternative Title | Sep. Sci. Technol. |
| CHANG, QL; CHEN, JY | |
| 1995 | |
| Source Publication | SEPARATION SCIENCE AND TECHNOLOGY
 |
| ISSN | 0149-6395 |
| Volume | 30Issue:13Pages:2679-2693 |
| Abstract | Seven kinds of alkyl alcohol (n-butanol, n-pentanol, n-hexanol, n-heptanol, n-octanol, n-nonanol, and n-decanol) were chosen as the cosolvent for Aliquat 336/ isooctane reversed micelles. By performing liquid-liquid extraction with Aliquat 336 reversed micelles as the extractant, the separation and purification of two enzymes (alpha-amylase and neutral protease) from Bacillus subtilis were investigated. Experiments revealed that when n-butanol is used as the cosolvent, the two enzymes from Bacillus subtilis can be effectively separated and purified after a full forward and backward extraction cycle. alpha-Amylase was separated in the stripping solution with about 85% of its total activity recovered and purified about 1.6-fold. Neutral protease was separated in the raffinate with about 80% of its total activity recovered and purified about 3.5-fold. For the other six alcohols used as the cosolvent for Aliquat 336/isooctane reversed micelles, separation was not achieved.; Seven kinds of alkyl alcohol (n-butanol, n-pentanol, n-hexanol, n-heptanol, n-octanol, n-nonanol, and n-decanol) were chosen as the cosolvent for Aliquat 336/ isooctane reversed micelles. By performing liquid-liquid extraction with Aliquat 336 reversed micelles as the extractant, the separation and purification of two enzymes (alpha-amylase and neutral protease) from Bacillus subtilis were investigated. Experiments revealed that when n-butanol is used as the cosolvent, the two enzymes from Bacillus subtilis can be effectively separated and purified after a full forward and backward extraction cycle. alpha-Amylase was separated in the stripping solution with about 85% of its total activity recovered and purified about 1.6-fold. Neutral protease was separated in the raffinate with about 80% of its total activity recovered and purified about 3.5-fold. For the other six alcohols used as the cosolvent for Aliquat 336/isooctane reversed micelles, separation was not achieved. |
| Keyword | Reversed Micelles Cosolvent Separation Alpha-amylase Neutral Protease |
| Subtype | Article |
| WOS Headings | Science & Technology ; Physical Sciences ; Technology |
| URL | 查看原文 |
| Indexed By | SCI |
| Language | 英语 |
| WOS Keyword | LIQUID-LIQUID-EXTRACTION ; ALPHA-CHYMOTRYPSIN ; SOLUBILIZATION ; RECOVERY |
| WOS Research Area | Chemistry ; Engineering |
| WOS Subject | Chemistry, Multidisciplinary ; Engineering, Chemical |
| WOS ID | WOS:A1995RL69100004 |
| Citation statistics | |
| Document Type | 期刊论文 |
| Version | 出版稿 |
| Identifier | http://ir.ipe.ac.cn/handle/122111/5901 |
| Collection | 研究所（批量导入） |
| Recommended Citation GB/T 7714 | CHANG, QL,CHEN, JY. EFFECT OF THE TYPE OF COSOLVENT ON THE EXTRACTION PROCESS FOR SEPARATION AND PURIFICATION OF 2 ENZYMES FROM BACILLUS-SUBTILIS USING ALIQUAT-336 REVERSED MICELLES[J]. SEPARATION SCIENCE AND TECHNOLOGY,1995,30(13):2679-2693. |
| APA | CHANG, QL,&CHEN, JY.(1995).EFFECT OF THE TYPE OF COSOLVENT ON THE EXTRACTION PROCESS FOR SEPARATION AND PURIFICATION OF 2 ENZYMES FROM BACILLUS-SUBTILIS USING ALIQUAT-336 REVERSED MICELLES.SEPARATION SCIENCE AND TECHNOLOGY,30(13),2679-2693. |
| MLA | CHANG, QL,et al."EFFECT OF THE TYPE OF COSOLVENT ON THE EXTRACTION PROCESS FOR SEPARATION AND PURIFICATION OF 2 ENZYMES FROM BACILLUS-SUBTILIS USING ALIQUAT-336 REVERSED MICELLES".SEPARATION SCIENCE AND TECHNOLOGY 30.13(1995):2679-2693. |
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| Effect of the Type o（581KB） | 限制开放 | CC BY-NC-SA | Application Full Text | |||
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