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Effects of surfactants on emulsification and secondary structure of lysozyme in aqueous solutions
Alternative TitleBiochem. Eng. J.
Liu, HZ; Yang, WJ; Chen, JY
1998-12-01
Source PublicationBIOCHEMICAL ENGINEERING JOURNAL
ISSN1369-703X
Volume2Issue:3Pages:187-196
AbstractThis paper reports our investigations on the secondary structure of lysozyme in aqueous solution with D2O and comparing systems emulsified with ionic and non-ionic amphiphiles, respectively. The opposite effect of concentration of surfactants on the lysozyme aqueous system was studied and the activity of lysozyme by the turbidimetric assay with different kinds of surfactant was compared. The effect of different surfactants on emulsification volume formed was observed and discussed. The different interactive patterns between lysozyme and ionic and non-ionic surfactants, such as sodium dodecyl sulfate (SDS), cetyl trimethyl ammonium bromide (CTAB) and Triton-X100 (Isooctoylphenoxypolyethoxy ethanol), respectively, was studied with FTIR. A simple relationship between the secondary structure and denaturation of protein is given for the emulsification of lysozyme in aqueous solutions. It is important for understanding the stabilization and avoiding the emulsions in the extraction process that the interactions between the proteins and surfactants which leads to the formation of such complexes are characterized. The utility of surfactants as a de-emulsifying agent for the commercial process to extract penicillin G from the filtrate of fermentation broth was consistent with these results. (C) 1998 Elsevier Science S.A. All rights reserved.; This paper reports our investigations on the secondary structure of lysozyme in aqueous solution with D2O and comparing systems emulsified with ionic and non-ionic amphiphiles, respectively. The opposite effect of concentration of surfactants on the lysozyme aqueous system was studied and the activity of lysozyme by the turbidimetric assay with different kinds of surfactant was compared. The effect of different surfactants on emulsification volume formed was observed and discussed. The different interactive patterns between lysozyme and ionic and non-ionic surfactants, such as sodium dodecyl sulfate (SDS), cetyl trimethyl ammonium bromide (CTAB) and Triton-X100 (Isooctoylphenoxypolyethoxy ethanol), respectively, was studied with FTIR. A simple relationship between the secondary structure and denaturation of protein is given for the emulsification of lysozyme in aqueous solutions. It is important for understanding the stabilization and avoiding the emulsions in the extraction process that the interactions between the proteins and surfactants which leads to the formation of such complexes are characterized. The utility of surfactants as a de-emulsifying agent for the commercial process to extract penicillin G from the filtrate of fermentation broth was consistent with these results. (C) 1998 Elsevier Science S.A. All rights reserved.
KeywordLysozyme Emulsification Ftir Surfactant
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Technology
URL查看原文
Indexed BySCI
Language英语
WOS KeywordPROTEIN CONFORMATION ; INFRARED-SPECTRA ; DECONVOLUTION
WOS Research AreaBiotechnology & Applied Microbiology ; Engineering
WOS SubjectBiotechnology & Applied Microbiology ; Engineering, Chemical
WOS IDWOS:000077894900003
Citation statistics
Cited Times:25[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Version出版稿
Identifierhttp://ir.ipe.ac.cn/handle/122111/5907
Collection研究所(批量导入)
AffiliationChinese Acad Sci, Inst Chem Met, Beijing 100080, Peoples R China
Recommended Citation
GB/T 7714
Liu, HZ,Yang, WJ,Chen, JY. Effects of surfactants on emulsification and secondary structure of lysozyme in aqueous solutions[J]. BIOCHEMICAL ENGINEERING JOURNAL,1998,2(3):187-196.
APA Liu, HZ,Yang, WJ,&Chen, JY.(1998).Effects of surfactants on emulsification and secondary structure of lysozyme in aqueous solutions.BIOCHEMICAL ENGINEERING JOURNAL,2(3),187-196.
MLA Liu, HZ,et al."Effects of surfactants on emulsification and secondary structure of lysozyme in aqueous solutions".BIOCHEMICAL ENGINEERING JOURNAL 2.3(1998):187-196.
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