Knowledge Management System Of Institute of process engineering,CAS
| Enhanced peroxidase production by suspension culture of carrot compact callus aggregates | |
| Alternative Title | J. Biotechnol. |
| Xu, JF; Sun, Y; Su, ZG | |
| 1998-10-27 | |
| Source Publication | JOURNAL OF BIOTECHNOLOGY
 |
| ISSN | 0168-1656 |
| Volume | 65Issue:2-3Pages:203-208 |
| Abstract | Suspension culture of compact callus aggregates (CCA) of Daucus carota (carrot), consisting of yellow, spherical cellular clumps displaying tissue differentiation was established. The production of peroxidase which was detected in trace amounts in dispersed carrot cell cultures was found in high amounts (5.0-14.5 U g(-1) FW) in CCA cultures. Kinetics analysis showed that CCA grew quickly for 4-12 days with a specific growth rate of 0.20 day(-1), while the peroxidase activity increased sharply after day 12. Fungal elicitors were found to effectively induce peroxidase synthesis. Peroxidase activity of 54.0 U g(-1) FW was obtained by adding Aspergillus niger elicitor at day 16 to make its concentration in medium 50 mg l(-1). (C) 1998 Elsevier Science B.V. All rights reserved.; Suspension culture of compact callus aggregates (CCA) of Daucus carota (carrot), consisting of yellow, spherical cellular clumps displaying tissue differentiation was established. The production of peroxidase which was detected in trace amounts in dispersed carrot cell cultures was found in high amounts (5.0-14.5 U g(-1) FW) in CCA cultures. Kinetics analysis showed that CCA grew quickly for 4-12 days with a specific growth rate of 0.20 day(-1), while the peroxidase activity increased sharply after day 12. Fungal elicitors were found to effectively induce peroxidase synthesis. Peroxidase activity of 54.0 U g(-1) FW was obtained by adding Aspergillus niger elicitor at day 16 to make its concentration in medium 50 mg l(-1). (C) 1998 Elsevier Science B.V. All rights reserved. |
| Keyword | Daucus Carota Peroxidase Plant Cell Culture Elicitor |
| Subtype | Article |
| WOS Headings | Science & Technology ; Life Sciences & Biomedicine |
| URL | 查看原文 |
| Indexed By | SCI |
| Language | 英语 |
| WOS Keyword | CELL-CULTURES ; GROWTH |
| WOS Research Area | Biotechnology & Applied Microbiology |
| WOS Subject | Biotechnology & Applied Microbiology |
| WOS ID | WOS:000077143100009 |
| Citation statistics | |
| Document Type | 期刊论文 |
| Version | 出版稿 |
| Identifier | http://ir.ipe.ac.cn/handle/122111/5917 |
| Collection | 研究所（批量导入） |
| Affiliation | 1.Chinese Acad Sci, Inst Chem Met, State Key Lab Biochem Engn, Beijing 100080, Peoples R China 2.China Agr Univ, Inst Fundamental Chem, Beijing 100094, Peoples R China |
| Recommended Citation GB/T 7714 | Xu, JF,Sun, Y,Su, ZG. Enhanced peroxidase production by suspension culture of carrot compact callus aggregates[J]. JOURNAL OF BIOTECHNOLOGY,1998,65(2-3):203-208. |
| APA | Xu, JF,Sun, Y,&Su, ZG.(1998).Enhanced peroxidase production by suspension culture of carrot compact callus aggregates.JOURNAL OF BIOTECHNOLOGY,65(2-3),203-208. |
| MLA | Xu, JF,et al."Enhanced peroxidase production by suspension culture of carrot compact callus aggregates".JOURNAL OF BIOTECHNOLOGY 65.2-3(1998):203-208. |
| Files in This Item: | ||||||
| File Name/Size | DocType | Version | Access | License | ||
| Enhanced peroxidase （283KB） | 限制开放 | CC BY-NC-SA | Application Full Text | |||
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