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Enhanced peroxidase production by suspension culture of carrot compact callus aggregates
Alternative TitleJ. Biotechnol.
Xu, JF; Sun, Y; Su, ZG
1998-10-27
Source PublicationJOURNAL OF BIOTECHNOLOGY
ISSN0168-1656
Volume65Issue:2-3Pages:203-208
AbstractSuspension culture of compact callus aggregates (CCA) of Daucus carota (carrot), consisting of yellow, spherical cellular clumps displaying tissue differentiation was established. The production of peroxidase which was detected in trace amounts in dispersed carrot cell cultures was found in high amounts (5.0-14.5 U g(-1) FW) in CCA cultures. Kinetics analysis showed that CCA grew quickly for 4-12 days with a specific growth rate of 0.20 day(-1), while the peroxidase activity increased sharply after day 12. Fungal elicitors were found to effectively induce peroxidase synthesis. Peroxidase activity of 54.0 U g(-1) FW was obtained by adding Aspergillus niger elicitor at day 16 to make its concentration in medium 50 mg l(-1). (C) 1998 Elsevier Science B.V. All rights reserved.; Suspension culture of compact callus aggregates (CCA) of Daucus carota (carrot), consisting of yellow, spherical cellular clumps displaying tissue differentiation was established. The production of peroxidase which was detected in trace amounts in dispersed carrot cell cultures was found in high amounts (5.0-14.5 U g(-1) FW) in CCA cultures. Kinetics analysis showed that CCA grew quickly for 4-12 days with a specific growth rate of 0.20 day(-1), while the peroxidase activity increased sharply after day 12. Fungal elicitors were found to effectively induce peroxidase synthesis. Peroxidase activity of 54.0 U g(-1) FW was obtained by adding Aspergillus niger elicitor at day 16 to make its concentration in medium 50 mg l(-1). (C) 1998 Elsevier Science B.V. All rights reserved.
KeywordDaucus Carota Peroxidase Plant Cell Culture Elicitor
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
URL查看原文
Indexed BySCI
Language英语
WOS KeywordCELL-CULTURES ; GROWTH
WOS Research AreaBiotechnology & Applied Microbiology
WOS SubjectBiotechnology & Applied Microbiology
WOS IDWOS:000077143100009
Citation statistics
Cited Times:8[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Version出版稿
Identifierhttp://ir.ipe.ac.cn/handle/122111/5917
Collection研究所(批量导入)
Affiliation1.Chinese Acad Sci, Inst Chem Met, State Key Lab Biochem Engn, Beijing 100080, Peoples R China
2.China Agr Univ, Inst Fundamental Chem, Beijing 100094, Peoples R China
Recommended Citation
GB/T 7714
Xu, JF,Sun, Y,Su, ZG. Enhanced peroxidase production by suspension culture of carrot compact callus aggregates[J]. JOURNAL OF BIOTECHNOLOGY,1998,65(2-3):203-208.
APA Xu, JF,Sun, Y,&Su, ZG.(1998).Enhanced peroxidase production by suspension culture of carrot compact callus aggregates.JOURNAL OF BIOTECHNOLOGY,65(2-3),203-208.
MLA Xu, JF,et al."Enhanced peroxidase production by suspension culture of carrot compact callus aggregates".JOURNAL OF BIOTECHNOLOGY 65.2-3(1998):203-208.
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