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Extraction of yeast alcohol dehydrogenase using reversed micelles formed with CTAB
Alternative TitleJ. Chem. Technol. Biotechnol.
Zhang, TX; Liu, HZ; Chen, JY
2000-09-01
Source PublicationJOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY
ISSN0268-2575
Volume75Issue:9Pages:798-802
AbstractA large molecular weight protein, alcohol dehydrogenase (ADH, 141 kDa), has been purified fi om baker's yeast using reversed micelles formed with a cationic surfactant (cetyltrimethylammonium bromide, CTAB) using the phase transfer method. Various parameters, such as pH, ionic strength and contact time of the two phases in ADH forward and backward transfer were studied. The active ADH was successfully recovered after a full forward and backward extraction cycle. The recovery of ADH activity obtained was similar to 90% and the purification factor was 3.1 for the overall process. (C) 2000 Society of Chemical Industry.; A large molecular weight protein, alcohol dehydrogenase (ADH, 141 kDa), has been purified fi om baker's yeast using reversed micelles formed with a cationic surfactant (cetyltrimethylammonium bromide, CTAB) using the phase transfer method. Various parameters, such as pH, ionic strength and contact time of the two phases in ADH forward and backward transfer were studied. The active ADH was successfully recovered after a full forward and backward extraction cycle. The recovery of ADH activity obtained was similar to 90% and the purification factor was 3.1 for the overall process. (C) 2000 Society of Chemical Industry.
KeywordReversed Micelles Alcohol Dehydrogenase Protein Purification Ctab
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Physical Sciences ; Technology
URL查看原文
Indexed BySCI
Language英语
WOS KeywordPROTEIN EXTRACTION ; PHOSPHORIC-ACID ; PURIFICATION ; ENZYMES
WOS Research AreaBiotechnology & Applied Microbiology ; Chemistry ; Engineering
WOS SubjectBiotechnology & Applied Microbiology ; Chemistry, Multidisciplinary ; Engineering, Chemical
WOS IDWOS:000088934100006
Citation statistics
Cited Times:17[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Version出版稿
Identifierhttp://ir.ipe.ac.cn/handle/122111/5935
Collection研究所(批量导入)
AffiliationChinese Acad Sci, Lab Separat Sci & Engn, Inst Chem Met, Beijing 100080, Peoples R China
Recommended Citation
GB/T 7714
Zhang, TX,Liu, HZ,Chen, JY. Extraction of yeast alcohol dehydrogenase using reversed micelles formed with CTAB[J]. JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY,2000,75(9):798-802.
APA Zhang, TX,Liu, HZ,&Chen, JY.(2000).Extraction of yeast alcohol dehydrogenase using reversed micelles formed with CTAB.JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY,75(9),798-802.
MLA Zhang, TX,et al."Extraction of yeast alcohol dehydrogenase using reversed micelles formed with CTAB".JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY 75.9(2000):798-802.
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