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Extractive cultivation of Lactococcus lactis using a polyethylene glycol/MgSO4 center dot 7H(2)O aqueous two-phase system to produce nisin
Alternative TitleBiotechnol. Lett.
Li, C; Fan, OY; Bai, JH
2000-05-01
Source PublicationBIOTECHNOLOGY LETTERS
ISSN0141-5492
Volume22Issue:10Pages:843-847
AbstractTo relieve lactic acid inhibition, an aqueous two-phase system (ATPS) was used to grow Lactococcus lactis. Its composition was 11% (w/v) PEG 20000/3.5% (w/v) MgSO4 . 7H(2)O. In this ATPS medium, the cells were completely partitioned in the bottom phase, and lactic acid had the biggest partition coefficient of the eight ATPS media tested. The cell biomass in this medium was 0.64 mg ml(-1), only 60% of that of the control medium, but nisin production (803 IU ml(-1)) was enhanced by 33%. The increase in nisin was explained as a result of extraction of lactic acid from the bottom phase to the top one. The changes of tie-line length and phase volume ratio for the identical tie line could affect cell growth and nisin accumulation.; To relieve lactic acid inhibition, an aqueous two-phase system (ATPS) was used to grow Lactococcus lactis. Its composition was 11% (w/v) PEG 20000/3.5% (w/v) MgSO4 . 7H(2)O. In this ATPS medium, the cells were completely partitioned in the bottom phase, and lactic acid had the biggest partition coefficient of the eight ATPS media tested. The cell biomass in this medium was 0.64 mg ml(-1), only 60% of that of the control medium, but nisin production (803 IU ml(-1)) was enhanced by 33%. The increase in nisin was explained as a result of extraction of lactic acid from the bottom phase to the top one. The changes of tie-line length and phase volume ratio for the identical tie line could affect cell growth and nisin accumulation.
KeywordAqueous Two-phase Systems Extractive Cultivation Lactic Acid L. L.ctis Nisin
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
URL查看原文
Indexed BySCI
Language英语
WOS Keyword2-PHASE SYSTEM ; ALKALINE PROTEASE ; ACID BACTERIA
WOS Research AreaBiotechnology & Applied Microbiology
WOS SubjectBiotechnology & Applied Microbiology
WOS IDWOS:000088034000006
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Cited Times:19[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Version出版稿
Identifierhttp://ir.ipe.ac.cn/handle/122111/5936
Collection研究所(批量导入)
Affiliation1.Chinese Acad Sci, Inst Chem Met, State Key Lab Biochem Engn, Beijing 100080, Peoples R China
2.Hubei Univ, Sch Life Sci, Wuhan 430062, Peoples R China
Recommended Citation
GB/T 7714
Li, C,Fan, OY,Bai, JH. Extractive cultivation of Lactococcus lactis using a polyethylene glycol/MgSO4 center dot 7H(2)O aqueous two-phase system to produce nisin[J]. BIOTECHNOLOGY LETTERS,2000,22(10):843-847.
APA Li, C,Fan, OY,&Bai, JH.(2000).Extractive cultivation of Lactococcus lactis using a polyethylene glycol/MgSO4 center dot 7H(2)O aqueous two-phase system to produce nisin.BIOTECHNOLOGY LETTERS,22(10),843-847.
MLA Li, C,et al."Extractive cultivation of Lactococcus lactis using a polyethylene glycol/MgSO4 center dot 7H(2)O aqueous two-phase system to produce nisin".BIOTECHNOLOGY LETTERS 22.10(2000):843-847.
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