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Flocculation and characterization of protein by anionic copolymer containing reactive functional groups
Alternative TitleColloid Surf. A-Physicochem. Eng. Asp.
Yu, J; Liu, HZ; Chen, JY
2000-04-15
Source PublicationCOLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
ISSN0927-7757
Volume163Issue:2-3Pages:225-232
AbstractTwo types of anionic copolymers with reactive functional groups of amido, carboxyl and sulfonate were prepared and used to study the flocculation and characterization of lysozyme in aqueous solution. Initial increase of the ionic strength promotes flocculation, but no flocculation occurs with further increase of ionic strength. High ionic strength in solution also makes the aggregates of protein-copolymer sensitive to sheaf. The two types of flocculants require high pH to achieve high charge density for interacting with lysozyme effectively. Adsorption of lysozyme to the anionic copolymers was influenced strongly by pH and ionic strength. The results reveal an understanding of the types of zeta (zeta) potential when lysozyme aggregates with copolymers. The performances of the anionic copolymers are related to their compositions when they are used as flocculants. The results confirm the mechanism of charge neutralization that prevails for highly charged polymers. (C) 2000 Elsevier Science B.V. All rights reserved.; Two types of anionic copolymers with reactive functional groups of amido, carboxyl and sulfonate were prepared and used to study the flocculation and characterization of lysozyme in aqueous solution. Initial increase of the ionic strength promotes flocculation, but no flocculation occurs with further increase of ionic strength. High ionic strength in solution also makes the aggregates of protein-copolymer sensitive to sheaf. The two types of flocculants require high pH to achieve high charge density for interacting with lysozyme effectively. Adsorption of lysozyme to the anionic copolymers was influenced strongly by pH and ionic strength. The results reveal an understanding of the types of zeta (zeta) potential when lysozyme aggregates with copolymers. The performances of the anionic copolymers are related to their compositions when they are used as flocculants. The results confirm the mechanism of charge neutralization that prevails for highly charged polymers. (C) 2000 Elsevier Science B.V. All rights reserved.
KeywordAnionic Copolymer Reactive Functional Group Flocculation Lysozyme
SubtypeArticle
WOS HeadingsScience & Technology ; Physical Sciences
URL查看原文
Indexed BySCI
Language英语
WOS KeywordSELECTIVE FLOCCULATION ; POLYELECTROLYTE ; SEPARATION ; CHITOSAN
WOS Research AreaChemistry
WOS SubjectChemistry, Physical
WOS IDWOS:000085374200010
Citation statistics
Cited Times:7[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Version出版稿
Identifierhttp://ir.ipe.ac.cn/handle/122111/5937
Collection研究所(批量导入)
AffiliationChinese Acad Sci, Inst Chem Met, Young Scientist Lab Separat Sci & Engn, Beijing 100080, Peoples R China
Recommended Citation
GB/T 7714
Yu, J,Liu, HZ,Chen, JY. Flocculation and characterization of protein by anionic copolymer containing reactive functional groups[J]. COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS,2000,163(2-3):225-232.
APA Yu, J,Liu, HZ,&Chen, JY.(2000).Flocculation and characterization of protein by anionic copolymer containing reactive functional groups.COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS,163(2-3),225-232.
MLA Yu, J,et al."Flocculation and characterization of protein by anionic copolymer containing reactive functional groups".COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS 163.2-3(2000):225-232.
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