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High-frequency plant regeneration via somatic embryogenesis and organogenesis and in vitro flowering of regenerated plantlets in Panax ginseng
Alternative TitlePlant Cell Reports
Tang, W
2000-06-01
Source PublicationPLANT CELL REPORTS
ISSN0721-7714
Volume19Issue:7Pages:727-732
AbstractThe morphogenesis ability of light yellowish globular callus derived from cotyledons of mature zygotic embryos of Panax ginseng was investigated. The optimal media for somatic embryogenesis and shoot organogenesis were MS medium containing 0.5 mg l(-1) 2.4-dichlorophenoxyacetic acid, 0.1 mg l(-1) 6-benzyladenine (BA), and 500 mg l(-1) lactoalbumin hydrolysate, and SH medium supplemented with 0.5 mg l(-1) alpha-naphthaleneacetic acid, 0.1 mg l(-1) BA, and 500 mg l(-1) casein hydrolysate. The influences of glucose, mannose, fructose, and sorbose in the media on somatic embryogenesis and shoot organogenesis were revealed as differences in the numbers of somatic embryos and adventitious shoots per gram of morphogenic callus. The best regeneration of somatic embryos was obtained on medium containing glucose, with a mean of 8.7 somatic embryos per gram of callus. The best regeneration of shoots was observed on medium containing fructose, with an average of 12.2 adventitious shoots per gram of callus. Of the somatic embryos 95% were converted into regenerated plantlets, and 100% of adventitious shoots rooted to form regenerated plantlets. Regenerated plants were successfully established in soil. Flowering was observed in 5.7% of the regenerated plants derived from shoot organogenesis and in 1.4% of the regenerated plants derived from somatic embryogenesis.; The morphogenesis ability of light yellowish globular callus derived from cotyledons of mature zygotic embryos of Panax ginseng was investigated. The optimal media for somatic embryogenesis and shoot organogenesis were MS medium containing 0.5 mg l(-1) 2.4-dichlorophenoxyacetic acid, 0.1 mg l(-1) 6-benzyladenine (BA), and 500 mg l(-1) lactoalbumin hydrolysate, and SH medium supplemented with 0.5 mg l(-1) alpha-naphthaleneacetic acid, 0.1 mg l(-1) BA, and 500 mg l(-1) casein hydrolysate. The influences of glucose, mannose, fructose, and sorbose in the media on somatic embryogenesis and shoot organogenesis were revealed as differences in the numbers of somatic embryos and adventitious shoots per gram of morphogenic callus. The best regeneration of somatic embryos was obtained on medium containing glucose, with a mean of 8.7 somatic embryos per gram of callus. The best regeneration of shoots was observed on medium containing fructose, with an average of 12.2 adventitious shoots per gram of callus. Of the somatic embryos 95% were converted into regenerated plantlets, and 100% of adventitious shoots rooted to form regenerated plantlets. Regenerated plants were successfully established in soil. Flowering was observed in 5.7% of the regenerated plants derived from shoot organogenesis and in 1.4% of the regenerated plants derived from somatic embryogenesis.
KeywordPanax Ginseng Ca Meyer Somatic Embryogenesis Organogenesis In Vitro flowerIng Of Regenerated Plantlets
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
URL查看原文
Indexed BySCI
Language英语
WOS KeywordCELL-CULTURES ; SAPONIN PRODUCTION ; EMBRYOS ; GROWTH
WOS Research AreaPlant Sciences
WOS SubjectPlant Sciences
WOS IDWOS:000088156600014
Citation statistics
Cited Times:27[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Version出版稿
Identifierhttp://ir.ipe.ac.cn/handle/122111/5956
Collection研究所(批量导入)
AffiliationChinese Acad Sci, Inst Chem Met, Beijing 100080, Peoples R China
Recommended Citation
GB/T 7714
Tang, W. High-frequency plant regeneration via somatic embryogenesis and organogenesis and in vitro flowering of regenerated plantlets in Panax ginseng[J]. PLANT CELL REPORTS,2000,19(7):727-732.
APA Tang, W.(2000).High-frequency plant regeneration via somatic embryogenesis and organogenesis and in vitro flowering of regenerated plantlets in Panax ginseng.PLANT CELL REPORTS,19(7),727-732.
MLA Tang, W."High-frequency plant regeneration via somatic embryogenesis and organogenesis and in vitro flowering of regenerated plantlets in Panax ginseng".PLANT CELL REPORTS 19.7(2000):727-732.
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