Knowledge Management System Of Institute of process engineering,CAS
| The interaction between Triton X-100 and bovine serum albumin | |
| Alternative Title | Chin. J. Anal. Chem. |
| Wei, XF; Liu, HZ | |
| 2000-06-20 | |
| Source Publication | CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
 |
| ISSN | 0253-3820 |
| Volume | 28Issue:6Pages:699-701 |
| Abstract | The binding of Triton X-100 (Tx) to bovine serum albumin(BSA) was studied using fluorescence spectroscopy. It was shown that TX has a strong ability to quench the BSA fluorescence mainly via a static quenching. The binding constant K and the number of bindig n were obtained from the fluorescence quenching results. It was revealed that K and n were changed with varying concentrations of TX: below cmc of TX, K was 440 mol/L, n was 0.91; above cmc, K was 10 mg/L, n was 0.42. The hydrophobic interactions played a major role in the binding process.; The binding of Triton X-100 (Tx) to bovine serum albumin(BSA) was studied using fluorescence spectroscopy. It was shown that TX has a strong ability to quench the BSA fluorescence mainly via a static quenching. The binding constant K and the number of bindig n were obtained from the fluorescence quenching results. It was revealed that K and n were changed with varying concentrations of TX: below cmc of TX, K was 440 mol/L, n was 0.91; above cmc, K was 10 mg/L, n was 0.42. The hydrophobic interactions played a major role in the binding process. |
| Keyword | Bovine Serum Albumin Fluorimetry |
| Subtype | Article |
| WOS Headings | Science & Technology ; Physical Sciences |
| URL | 查看原文 |
| Indexed By | SCI |
| Language | 英语 |
| WOS Keyword | PROTEIN FLUORESCENCE |
| WOS Research Area | Chemistry |
| WOS Subject | Chemistry, Analytical |
| WOS ID | WOS:000087852500009 |
| Citation statistics | |
| Document Type | 期刊论文 |
| Version | 出版稿 |
| Identifier | http://ir.ipe.ac.cn/handle/122111/5969 |
| Collection | 研究所（批量导入） |
| Affiliation | Chinese Acad Sci, Inst Chem Met, Young Sci Lab Separat Sci & Engn, Beijing 100080, Peoples R China |
| Recommended Citation GB/T 7714 | Wei, XF,Liu, HZ. The interaction between Triton X-100 and bovine serum albumin[J]. CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,2000,28(6):699-701. |
| APA | Wei, XF,&Liu, HZ.(2000).The interaction between Triton X-100 and bovine serum albumin.CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,28(6),699-701. |
| MLA | Wei, XF,et al."The interaction between Triton X-100 and bovine serum albumin".CHINESE JOURNAL OF ANALYTICAL CHEMISTRY 28.6(2000):699-701. |
| Files in This Item: | ||||||
| File Name/Size | DocType | Version | Access | License | ||
| TritonX_100与牛血清白蛋白的相（203KB） | 限制开放 | CC BY-NC-SA | Application Full Text | |||
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