CAS OpenIR  > 研究所(批量导入)
The interaction between Triton X-100 and bovine serum albumin
Alternative TitleChin. J. Anal. Chem.
Wei, XF; Liu, HZ
2000-06-20
Source PublicationCHINESE JOURNAL OF ANALYTICAL CHEMISTRY
ISSN0253-3820
Volume28Issue:6Pages:699-701
AbstractThe binding of Triton X-100 (Tx) to bovine serum albumin(BSA) was studied using fluorescence spectroscopy. It was shown that TX has a strong ability to quench the BSA fluorescence mainly via a static quenching. The binding constant K and the number of bindig n were obtained from the fluorescence quenching results. It was revealed that K and n were changed with varying concentrations of TX: below cmc of TX, K was 440 mol/L, n was 0.91; above cmc, K was 10 mg/L, n was 0.42. The hydrophobic interactions played a major role in the binding process.; The binding of Triton X-100 (Tx) to bovine serum albumin(BSA) was studied using fluorescence spectroscopy. It was shown that TX has a strong ability to quench the BSA fluorescence mainly via a static quenching. The binding constant K and the number of bindig n were obtained from the fluorescence quenching results. It was revealed that K and n were changed with varying concentrations of TX: below cmc of TX, K was 440 mol/L, n was 0.91; above cmc, K was 10 mg/L, n was 0.42. The hydrophobic interactions played a major role in the binding process.
KeywordBovine Serum Albumin Fluorimetry
SubtypeArticle
WOS HeadingsScience & Technology ; Physical Sciences
URL查看原文
Indexed BySCI
Language英语
WOS KeywordPROTEIN FLUORESCENCE
WOS Research AreaChemistry
WOS SubjectChemistry, Analytical
WOS IDWOS:000087852500009
Citation statistics
Cited Times:48[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Version出版稿
Identifierhttp://ir.ipe.ac.cn/handle/122111/5969
Collection研究所(批量导入)
AffiliationChinese Acad Sci, Inst Chem Met, Young Sci Lab Separat Sci & Engn, Beijing 100080, Peoples R China
Recommended Citation
GB/T 7714
Wei, XF,Liu, HZ. The interaction between Triton X-100 and bovine serum albumin[J]. CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,2000,28(6):699-701.
APA Wei, XF,&Liu, HZ.(2000).The interaction between Triton X-100 and bovine serum albumin.CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,28(6),699-701.
MLA Wei, XF,et al."The interaction between Triton X-100 and bovine serum albumin".CHINESE JOURNAL OF ANALYTICAL CHEMISTRY 28.6(2000):699-701.
Files in This Item:
File Name/Size DocType Version Access License
TritonX_100与牛血清白蛋白的相(203KB) 限制开放CC BY-NC-SAApplication Full Text
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Wei, XF]'s Articles
[Liu, HZ]'s Articles
Baidu academic
Similar articles in Baidu academic
[Wei, XF]'s Articles
[Liu, HZ]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Wei, XF]'s Articles
[Liu, HZ]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.