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The investigation for lysozyme conformation changes in deuteromethanol solution by FT-Raman spectrometer
Alternative TitleSpectrosc. Spectr. Anal.
Wang, B; Wang, J; Yu, J; Liu, HZ
1999-08-01
Source PublicationSPECTROSCOPY AND SPECTRAL ANALYSIS
ISSN1000-0593
Volume19Issue:4Pages:535-537
AbstractLysozyme conformation in deuteromethanol-deuteroxide solution has been studied by FT-Raman spectrometer. By using amide I mode in the Raman spectra and curve fitting method, information of Lysozyme's secondary structure is easily detected in details, and tertiary structure changes can be suggested by relative intensity changes of some bands. It is concluded that lysozyme conformation may be changed to loose structure with incrasing deuteromethanol concentration, and it seems like that increasing loose beta-sheet content lead to the gel's appearing in the solution.; Lysozyme conformation in deuteromethanol-deuteroxide solution has been studied by FT-Raman spectrometer. By using amide I mode in the Raman spectra and curve fitting method, information of Lysozyme's secondary structure is easily detected in details, and tertiary structure changes can be suggested by relative intensity changes of some bands. It is concluded that lysozyme conformation may be changed to loose structure with incrasing deuteromethanol concentration, and it seems like that increasing loose beta-sheet content lead to the gel's appearing in the solution.
KeywordFt-raman Lysozyme Conformation Deuteromethanol
SubtypeArticle
WOS HeadingsScience & Technology ; Technology
URL查看原文
Indexed BySCI
Language英语
WOS KeywordSECONDARY STRUCTURE ; PROTEINS ; DECONVOLUTION ; BAND
WOS Research AreaSpectroscopy
WOS SubjectSpectroscopy
WOS IDWOS:000084127900007
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Cited Times:2[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Version出版稿
Identifierhttp://ir.ipe.ac.cn/handle/122111/5971
Collection研究所(批量导入)
AffiliationChinese Acad Sci, Inst Chem Met, Beijing 100080, Peoples R China
Recommended Citation
GB/T 7714
Wang, B,Wang, J,Yu, J,et al. The investigation for lysozyme conformation changes in deuteromethanol solution by FT-Raman spectrometer[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS,1999,19(4):535-537.
APA Wang, B,Wang, J,Yu, J,&Liu, HZ.(1999).The investigation for lysozyme conformation changes in deuteromethanol solution by FT-Raman spectrometer.SPECTROSCOPY AND SPECTRAL ANALYSIS,19(4),535-537.
MLA Wang, B,et al."The investigation for lysozyme conformation changes in deuteromethanol solution by FT-Raman spectrometer".SPECTROSCOPY AND SPECTRAL ANALYSIS 19.4(1999):535-537.
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