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Kinetic and technical studies on large-scale culture of Rhodiola sachalinensis compact callus aggregates with air-lift reactors
Alternative TitleJ. Chem. Technol. Biotechnol.
Xu, J. F.; Xie, J.; Han, A. M.; Feng, P. S.; Su, Z. G.
1998
Source PublicationJournal of Chemical Technology and Biotechnology
Issue3Pages:227-234
AbstractCompact callus aggregates (CCA) of Rhodiola sachalinensis, spherical, smooth-surfaced clumps of 2-8 mm in diameter displaying some level of tissue differentiation, were successfully cultured in 10 dm(3) and 100 dm(3) air-lift reactors. High salidroside yields of 60.0 mg dm(-3) were obtained, which were 10-fold the dispersed cell cultures. The salidroside accumulation was found to be growth-associated due to the differentiated structure of CCA. No 'foaming' was observed since the broth remained almost clear throughout the culture cycle. The size of CCA conformed to normal distribution with average diameters varying from 3.1 mm to 3.6 mm during the culture. The depositing velocity of CCA in culture broth was small enough to be readily retained in suspension, therefore avoiding the clogging of the reactors. The significant increase in solid hold-up of the culture system was suggested to contribute to the variation of k(L) a during the culture. (C) 1998 SCI.
KeywordRhodiola Sachalinensis Salidroside Plant Cell Culture Aggregates Air-lift Reactor Oxygen Transfer Coefficient Plant-cell Cultures Tissue-cultures Growth Accumulation Root
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Indexed BySCI
Language英语
Document Type期刊论文
Version出版稿
Identifierhttp://ir.ipe.ac.cn/handle/122111/5974
Collection研究所(批量导入)
Recommended Citation
GB/T 7714
Xu, J. F.,Xie, J.,Han, A. M.,et al. Kinetic and technical studies on large-scale culture of Rhodiola sachalinensis compact callus aggregates with air-lift reactors[J]. Journal of Chemical Technology and Biotechnology,1998(3):227-234.
APA Xu, J. F.,Xie, J.,Han, A. M.,Feng, P. S.,&Su, Z. G..(1998).Kinetic and technical studies on large-scale culture of Rhodiola sachalinensis compact callus aggregates with air-lift reactors.Journal of Chemical Technology and Biotechnology(3),227-234.
MLA Xu, J. F.,et al."Kinetic and technical studies on large-scale culture of Rhodiola sachalinensis compact callus aggregates with air-lift reactors".Journal of Chemical Technology and Biotechnology .3(1998):227-234.
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