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KINETIC-STUDY ON STARCH HYDROLYSIS USING A GLUCOSE MALTOSE SENSING SYSTEM
Alternative TitleBiotechnol. Tech.
QU, HB; ZHANG, XE; CHUI, WW; ZHANG, SZ; LI, GX; FAN, OY
1995-06-01
Source PublicationBIOTECHNOLOGY TECHNIQUES
ISSN0951-208X
Volume9Issue:6Pages:445-450
AbstractA dual-enzyme electrode flow injection system that can simultaneously determine glucose and maltose is used for an on-line study of starch hydrolyses catalysed by amylases. With the working system, determinations can be made every 2 minutes. A 10 mu L sample size with recycled back-flow minimises any loss of the reaction medium. The production, growth and decay of glucose and maltose concentrations during starch hydrolysis under various enzymatic conditions can thus be closely monitored, making it useful for the study of the catalytic kinetics of amylases and in screening and analysing enzyme systems.; A dual-enzyme electrode flow injection system that can simultaneously determine glucose and maltose is used for an on-line study of starch hydrolyses catalysed by amylases. With the working system, determinations can be made every 2 minutes. A 10 mu L sample size with recycled back-flow minimises any loss of the reaction medium. The production, growth and decay of glucose and maltose concentrations during starch hydrolysis under various enzymatic conditions can thus be closely monitored, making it useful for the study of the catalytic kinetics of amylases and in screening and analysing enzyme systems.
KeywordUltrafiltration Reactor Alpha-amylase
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
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Indexed BySCI
Language英语
WOS KeywordULTRAFILTRATION REACTOR ; ALPHA-AMYLASE
WOS Research AreaBiochemistry & Molecular Biology ; Biotechnology & Applied Microbiology
WOS SubjectBiochemical Research Methods ; Biotechnology & Applied Microbiology
WOS IDWOS:A1995RH40500013
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Document Type期刊论文
Version出版稿
Identifierhttp://ir.ipe.ac.cn/handle/122111/5981
Collection研究所(批量导入)
Affiliation1.CHINESE ACAD SCI,INST MICROBIOL,BEIJING 100080,PEOPLES R CHINA
2.CHINESE ACAD SCI,INST CHEM MET,NATL LAB BIOCHEM ENGN,BEIJING 100080,PEOPLES R CHINA
Recommended Citation
GB/T 7714
QU, HB,ZHANG, XE,CHUI, WW,et al. KINETIC-STUDY ON STARCH HYDROLYSIS USING A GLUCOSE MALTOSE SENSING SYSTEM[J]. BIOTECHNOLOGY TECHNIQUES,1995,9(6):445-450.
APA QU, HB,ZHANG, XE,CHUI, WW,ZHANG, SZ,LI, GX,&FAN, OY.(1995).KINETIC-STUDY ON STARCH HYDROLYSIS USING A GLUCOSE MALTOSE SENSING SYSTEM.BIOTECHNOLOGY TECHNIQUES,9(6),445-450.
MLA QU, HB,et al."KINETIC-STUDY ON STARCH HYDROLYSIS USING A GLUCOSE MALTOSE SENSING SYSTEM".BIOTECHNOLOGY TECHNIQUES 9.6(1995):445-450.
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