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Liquid-liquid reversed micellar extraction for isolating enzymes: Studies on the purification of alpha-amylase
Alternative TitleProcess Biochem.
Chang, QL; Chen, JY
1996-05-01
Source PublicationPROCESS BIOCHEMISTRY
ISSN1359-5113
Volume31Issue:4Pages:371-375
AbstractThe purification of alpha-amylase from a elude enzyme preparation was carried out by liquid-liquid reversed micellar extraction. After a full forward and background extraction cycle, the alpha-amylase was purified about 1.5-fold with 85% recovery. Most of the contaminating neutral protease in the crude enzyme preparation could be removed at the end of an extraction cycle. The separation factor of alpha-amylase to neutral protease reached about 10.; The purification of alpha-amylase from a elude enzyme preparation was carried out by liquid-liquid reversed micellar extraction. After a full forward and background extraction cycle, the alpha-amylase was purified about 1.5-fold with 85% recovery. Most of the contaminating neutral protease in the crude enzyme preparation could be removed at the end of an extraction cycle. The separation factor of alpha-amylase to neutral protease reached about 10.
KeywordSolubilization Recovery
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Technology
URL查看原文
Indexed BySCI
Language英语
WOS KeywordSOLUBILIZATION ; RECOVERY
WOS Research AreaBiochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Engineering
WOS SubjectBiochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Engineering, Chemical
WOS IDWOS:A1996TY83500008
Citation statistics
Cited Times:4[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Version出版稿
Identifierhttp://ir.ipe.ac.cn/handle/122111/5985
Collection研究所(批量导入)
Recommended Citation
GB/T 7714
Chang, QL,Chen, JY. Liquid-liquid reversed micellar extraction for isolating enzymes: Studies on the purification of alpha-amylase[J]. PROCESS BIOCHEMISTRY,1996,31(4):371-375.
APA Chang, QL,&Chen, JY.(1996).Liquid-liquid reversed micellar extraction for isolating enzymes: Studies on the purification of alpha-amylase.PROCESS BIOCHEMISTRY,31(4),371-375.
MLA Chang, QL,et al."Liquid-liquid reversed micellar extraction for isolating enzymes: Studies on the purification of alpha-amylase".PROCESS BIOCHEMISTRY 31.4(1996):371-375.
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