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"Modified flocculation" of protein with a combination of copolymer and polyacrylamide
Alternative TitleChem. Eng. Sci.
Yu, J; Liu, HZ; Chen, JY
1999-12-01
Source PublicationCHEMICAL ENGINEERING SCIENCE
ISSN0009-2509
Volume54Issue:24Pages:5839-5843
AbstractPrecipitation of soluble proteins in fermentation broth with addition of water-soluble polymers is an important method for downstream processing in biochemical technology. In the present work, the chemical features of "modified flocculation" are explored with lysozyme as the model protein to be precipitated with polyacrylamide in combination of an anionic copolymer containing reactive functional groups. The protein first interacts with low molecular weight anionic copolymer at gentle agitation, followed by precipitation with addition of high molecular weight polyacrylamide. The data on protein recovery in terms of light absorbance (280 nm) are analyzed to shed light on the mechanism of flee formation. The size distribution of the flocs was determined and the effects of the structure of the copolymer on the mechanism of flocculation were investigated. The results support the description of "modified flocculation", which enriches the interaction between protein and polymer, and leads to formation of protein-polymer flocs large enough to precipitate easily in water. (C) 1999 Elsevier Science Ltd. All rights reserved.; Precipitation of soluble proteins in fermentation broth with addition of water-soluble polymers is an important method for downstream processing in biochemical technology. In the present work, the chemical features of "modified flocculation" are explored with lysozyme as the model protein to be precipitated with polyacrylamide in combination of an anionic copolymer containing reactive functional groups. The protein first interacts with low molecular weight anionic copolymer at gentle agitation, followed by precipitation with addition of high molecular weight polyacrylamide. The data on protein recovery in terms of light absorbance (280 nm) are analyzed to shed light on the mechanism of flee formation. The size distribution of the flocs was determined and the effects of the structure of the copolymer on the mechanism of flocculation were investigated. The results support the description of "modified flocculation", which enriches the interaction between protein and polymer, and leads to formation of protein-polymer flocs large enough to precipitate easily in water. (C) 1999 Elsevier Science Ltd. All rights reserved.
KeywordFlocculation Protein Anionic Copolymer Polyacrylamide
SubtypeArticle
WOS HeadingsScience & Technology ; Technology
URL查看原文
Indexed BySCI
Language英语
WOS KeywordPRECIPITATION ; MECHANISM
WOS Research AreaEngineering
WOS SubjectEngineering, Chemical
WOS IDWOS:000082678100004
Citation statistics
Cited Times:5[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Version出版稿
Identifierhttp://ir.ipe.ac.cn/handle/122111/5996
Collection研究所(批量导入)
AffiliationChinese Acad Sci, Young Scientist Lab Separat Sci & Engn, Inst Chem Met, Beijing 100080, Peoples R China
Recommended Citation
GB/T 7714
Yu, J,Liu, HZ,Chen, JY. "Modified flocculation" of protein with a combination of copolymer and polyacrylamide[J]. CHEMICAL ENGINEERING SCIENCE,1999,54(24):5839-5843.
APA Yu, J,Liu, HZ,&Chen, JY.(1999)."Modified flocculation" of protein with a combination of copolymer and polyacrylamide.CHEMICAL ENGINEERING SCIENCE,54(24),5839-5843.
MLA Yu, J,et al.""Modified flocculation" of protein with a combination of copolymer and polyacrylamide".CHEMICAL ENGINEERING SCIENCE 54.24(1999):5839-5843.
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