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New solid-state fermentation process for repeated batch production of fibrinolytic enzyme by Fusarium oxysporum
Alternative TitleProcess Biochem.
Sun, T; Liu, BH; Li, P; Liu, DM; Li, ZH
1998-03-01
Source PublicationPROCESS BIOCHEMISTRY
ISSN1359-5113
Volume33Issue:4Pages:419-422
AbstractA novel solid-state fermentation system using rice chaff as carrier/substrate impregnated with mineral solution was developed in order to produce, repeatedly, an industrially useful enzyme. Fusarium oxysporum and a fibrinolytic enzyme was used as the model microorganism and target product, respectively. The dry spores were pneumatically inoculated and incubated for 96 hours, resulting in immobilized F. oxysporum cells on moist substrate. The ability of the immobilized culture to perform a long-term operation was confirmed by repeated batch fermentation. Production of the enzyme continued for 31.5 days and total fibrinolytic enzyme activity reached 154500 IU/litre bulk volume. (C) 1998 Elsevier Science Ltd. All rights reserved.; A novel solid-state fermentation system using rice chaff as carrier/substrate impregnated with mineral solution was developed in order to produce, repeatedly, an industrially useful enzyme. Fusarium oxysporum and a fibrinolytic enzyme was used as the model microorganism and target product, respectively. The dry spores were pneumatically inoculated and incubated for 96 hours, resulting in immobilized F. oxysporum cells on moist substrate. The ability of the immobilized culture to perform a long-term operation was confirmed by repeated batch fermentation. Production of the enzyme continued for 31.5 days and total fibrinolytic enzyme activity reached 154500 IU/litre bulk volume. (C) 1998 Elsevier Science Ltd. All rights reserved.
KeywordSolid State Fermentation Fibrinolytic Enzyme Fusarium Oxysporum
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Technology
URL查看原文
Indexed BySCI
Language英语
WOS KeywordGASEOUS CONCENTRATION GRADIENTS ; FERMENTERS ; CARRIER
WOS Research AreaBiochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Engineering
WOS SubjectBiochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Engineering, Chemical
WOS IDWOS:000073894300007
Citation statistics
Cited Times:3[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Version出版稿
Identifierhttp://ir.ipe.ac.cn/handle/122111/6003
Collection研究所(批量导入)
AffiliationChinese Acad Sci, Inst Chem Met, State Key Lab Biochem Engn, Beijing 100080, Peoples R China
Recommended Citation
GB/T 7714
Sun, T,Liu, BH,Li, P,et al. New solid-state fermentation process for repeated batch production of fibrinolytic enzyme by Fusarium oxysporum[J]. PROCESS BIOCHEMISTRY,1998,33(4):419-422.
APA Sun, T,Liu, BH,Li, P,Liu, DM,&Li, ZH.(1998).New solid-state fermentation process for repeated batch production of fibrinolytic enzyme by Fusarium oxysporum.PROCESS BIOCHEMISTRY,33(4),419-422.
MLA Sun, T,et al."New solid-state fermentation process for repeated batch production of fibrinolytic enzyme by Fusarium oxysporum".PROCESS BIOCHEMISTRY 33.4(1998):419-422.
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