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Plant regeneration through organogenesis from callus induced from mature zygotic embryos of loblolly pine
Alternative TitlePlant Cell Reports
Tang, W; Ouyang, F; Guo, ZC
1998-04-01
Source PublicationPLANT CELL REPORTS
ISSN0721-7714
Volume17Issue:6-7Pages:557-560
AbstractMature zygotic embryos from three seed sources of loblolly pine were cultured on callus induction medium containing 10 mg l(-1) alpha-naphthaleneacetic acid, 4 mg l(-1) benzyladenine (BA), 400 mg l(-1) casein hydrolysate, and 400 mg l(-1) glutamine for 6 weeks. Light-yellow, loose, glossy, globular callus was formed, and the highest frequency was 35.7%. The highest differentiation frequency of callus on adventitious bud induction medium was 62.1%. After culture of calli with adventitious buds on elongation medium for 6 weeks, adventitious shoots more than 1.0 cm in height were selected for rooting. On rooting medium supplemented with 0.1 mg l(-1) indole-3-butyric acid, 1 mg l(-1) BA, and 0.5 mg l(-1) gibberellic acid, the highest rooting frequency of adventitious shoots was 46% in a culture period of 6 weeks. Established plants survived following transfer to soil at a frequency of 71%.; Mature zygotic embryos from three seed sources of loblolly pine were cultured on callus induction medium containing 10 mg l(-1) alpha-naphthaleneacetic acid, 4 mg l(-1) benzyladenine (BA), 400 mg l(-1) casein hydrolysate, and 400 mg l(-1) glutamine for 6 weeks. Light-yellow, loose, glossy, globular callus was formed, and the highest frequency was 35.7%. The highest differentiation frequency of callus on adventitious bud induction medium was 62.1%. After culture of calli with adventitious buds on elongation medium for 6 weeks, adventitious shoots more than 1.0 cm in height were selected for rooting. On rooting medium supplemented with 0.1 mg l(-1) indole-3-butyric acid, 1 mg l(-1) BA, and 0.5 mg l(-1) gibberellic acid, the highest rooting frequency of adventitious shoots was 46% in a culture period of 6 weeks. Established plants survived following transfer to soil at a frequency of 71%.
KeywordPinus Taeda L. Organogenesis Plant Regeneration
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
URL查看原文
Indexed BySCI
Language英语
WOS KeywordABSCISIC-ACID ; CULTURES ; TAEDA
WOS Research AreaPlant Sciences
WOS SubjectPlant Sciences
WOS IDWOS:000073270200021
Citation statistics
Cited Times:24[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Version出版稿
Identifierhttp://ir.ipe.ac.cn/handle/122111/6019
Collection研究所(批量导入)
Affiliation1.Chinese Acad Sci, Inst Chem Met, State Key Lab Biochem Engn, Beijing 100080, Peoples R China
2.Chinese Acad Sci, Inst Bot, Beijing 100093, Peoples R China
Recommended Citation
GB/T 7714
Tang, W,Ouyang, F,Guo, ZC. Plant regeneration through organogenesis from callus induced from mature zygotic embryos of loblolly pine[J]. PLANT CELL REPORTS,1998,17(6-7):557-560.
APA Tang, W,Ouyang, F,&Guo, ZC.(1998).Plant regeneration through organogenesis from callus induced from mature zygotic embryos of loblolly pine.PLANT CELL REPORTS,17(6-7),557-560.
MLA Tang, W,et al."Plant regeneration through organogenesis from callus induced from mature zygotic embryos of loblolly pine".PLANT CELL REPORTS 17.6-7(1998):557-560.
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