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Polyethylene glycol improves the purification of recombinant human tumor necrosis factor during ion exchange chromatography
Alternative TitleBiotechnol. Tech.
Feng, XL; Gu, ZY; Jin, YT; Su, ZG
1998-04-01
Source PublicationBIOTECHNOLOGY TECHNIQUES
ISSN0951-208X
Volume12Issue:4Pages:289-293
AbstractPoly(ethylene glycol) (PEG) - either PEG 200, 600 or 4000 - when added to the buffer and feed solution gave nearly one-fold increase in the purification and about 100% recovery of recombinant human tumor necrosis factor-alpha during anion-exchange chromatography. The optimum concentration was 1% with the best result being achieved with PEG 200.; Poly(ethylene glycol) (PEG) - either PEG 200, 600 or 4000 - when added to the buffer and feed solution gave nearly one-fold increase in the purification and about 100% recovery of recombinant human tumor necrosis factor-alpha during anion-exchange chromatography. The optimum concentration was 1% with the best result being achieved with PEG 200.
KeywordProteins
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
URL查看原文
Indexed BySCI
Language英语
WOS KeywordPROTEINS
WOS Research AreaBiochemistry & Molecular Biology ; Biotechnology & Applied Microbiology
WOS SubjectBiochemical Research Methods ; Biotechnology & Applied Microbiology
WOS IDWOS:000073936700005
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Cited Times:5[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Version出版稿
Identifierhttp://ir.ipe.ac.cn/handle/122111/6021
Collection研究所(批量导入)
Affiliation1.Dalian Univ Technol, Sch Chem Engn, Biochem Engn Lab, Dalian 116012, Peoples R China
2.Chinese Acad Sci, Inst Chem Met, State Key Lab Biochem Engn, Beijing 100080, Peoples R China
Recommended Citation
GB/T 7714
Feng, XL,Gu, ZY,Jin, YT,et al. Polyethylene glycol improves the purification of recombinant human tumor necrosis factor during ion exchange chromatography[J]. BIOTECHNOLOGY TECHNIQUES,1998,12(4):289-293.
APA Feng, XL,Gu, ZY,Jin, YT,&Su, ZG.(1998).Polyethylene glycol improves the purification of recombinant human tumor necrosis factor during ion exchange chromatography.BIOTECHNOLOGY TECHNIQUES,12(4),289-293.
MLA Feng, XL,et al."Polyethylene glycol improves the purification of recombinant human tumor necrosis factor during ion exchange chromatography".BIOTECHNOLOGY TECHNIQUES 12.4(1998):289-293.
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