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Process integration of cell disruption and aqueous two-phase extraction
Alternative TitleJ. Chem. Technol. Biotechnol.
Su, ZG; Feng, XL
1999-03-01
Source PublicationJOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY
ISSN0268-2575
Volume74Issue:3Pages:284-288
AbstractA novel process was developed for extraction of intracellular proteins by combination of cell disruption and aqueous two-phase extraction in a single processing step. A horizontally stirred bead mill was used to perform the cell disruption and the protein extraction simultaneously without using an additional extractor. Polyethylene glycol and ammonium sulfate were added to the cell suspension before disruption. The bead mill worked both as a cell disrupter and as an extractor. The resulting homogenate after disruption was directly centrifuged for phase separation. The target protein was partitioned into the top phase while the cell debris was retained in the bottom phase. Separation of alcohol dehydrogenase (ADH) from yeast and human tumour necrosis factor alpha (cr-TNF) from recombinant Escherichia coli were tested as two model proteins in the new process. The ADH activity was increased in the presence of aqueous two-phase components. The TNF activity was also found to increase in the presence of PEG. This novel process has a good potential for separation of other intracellular biological products. (C) 1999 Society of Chemical Industry.; A novel process was developed for extraction of intracellular proteins by combination of cell disruption and aqueous two-phase extraction in a single processing step. A horizontally stirred bead mill was used to perform the cell disruption and the protein extraction simultaneously without using an additional extractor. Polyethylene glycol and ammonium sulfate were added to the cell suspension before disruption. The bead mill worked both as a cell disrupter and as an extractor. The resulting homogenate after disruption was directly centrifuged for phase separation. The target protein was partitioned into the top phase while the cell debris was retained in the bottom phase. Separation of alcohol dehydrogenase (ADH) from yeast and human tumour necrosis factor alpha (cr-TNF) from recombinant Escherichia coli were tested as two model proteins in the new process. The ADH activity was increased in the presence of aqueous two-phase components. The TNF activity was also found to increase in the presence of PEG. This novel process has a good potential for separation of other intracellular biological products. (C) 1999 Society of Chemical Industry.
KeywordCell Disruption Aqueous Two-phase Extraction Protein Purification Tumour Necrosis Factor Alcohol Dehydrogenase
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Physical Sciences ; Technology
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Indexed ByISTP ; SCI
Language英语
WOS KeywordPROTEIN
WOS Research AreaBiotechnology & Applied Microbiology ; Chemistry ; Engineering
WOS SubjectBiotechnology & Applied Microbiology ; Chemistry, Multidisciplinary ; Engineering, Chemical
WOS IDWOS:000079209400018
Citation statistics
Cited Times:11[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Version出版稿
Identifierhttp://ir.ipe.ac.cn/handle/122111/6031
Collection研究所(批量导入)
Affiliation1.Chinese Acad Sci, Inst Chem Met, State Key Lab Biochem Engn China, Beijing 100080, Peoples R China
2.Dalian Univ Technol, Dept Chem Engn, Dalian 116012, Peoples R China
Recommended Citation
GB/T 7714
Su, ZG,Feng, XL. Process integration of cell disruption and aqueous two-phase extraction[J]. JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY,1999,74(3):284-288.
APA Su, ZG,&Feng, XL.(1999).Process integration of cell disruption and aqueous two-phase extraction.JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY,74(3),284-288.
MLA Su, ZG,et al."Process integration of cell disruption and aqueous two-phase extraction".JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY 74.3(1999):284-288.
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