Knowledge Management System Of Institute of process engineering,CAS
| Production of salidroside through biotransformation of exogenous tyrosol in Rhodiola sachalinensis cell suspension cultures | |
| Alternative Title | Acta Bot. Sin. |
| Xu, JF; Su, ZG; Feng, PS | |
| 1998 | |
| Source Publication | ACTA BOTANICA SINICA
 |
| ISSN | 0577-7496 |
| Volume | 40Issue:12Pages:1129-1135 |
| Abstract | In Rhodiola sachalinensis A. Bor. cell cultures, low yields of salidroside was supposed to be associated with the low efficiency of glucosylation reaction at the stationary phase of cell growth, when large amounts of the substrate, aglycon tyrosol, were accumulated. Considering the activity of tyrosol glucosyltransferase being the highest at the exponential growth phase, the author added exogenous tyrosol into the cultures at this time so as to produce salidroside through biotransformation. The effects of tyrosol concentration, the way of tyrosol addition as well as the cell density on the transformation rate and salidroside yield were investigated. It was found that the transformation rate attained 95% after cells were incubated in the medium containing 1 mmol/L tyrosol for 24 h. Excess high concentrations of tyrosol in medium (> 3 mmol/L) caused inhibition of transformation rate and cell growth. By 3 repeated additions of tyrosol in low concentrations, the salidroside yields of 1 320 mg/L, 1 740 mg/L and 1 980 mg/L to the cell densities of 6 g DW/L, 12 g DW/L and 18 g DW/L were obtained respectively.; In Rhodiola sachalinensis A. Bor. cell cultures, low yields of salidroside was supposed to be associated with the low efficiency of glucosylation reaction at the stationary phase of cell growth, when large amounts of the substrate, aglycon tyrosol, were accumulated. Considering the activity of tyrosol glucosyltransferase being the highest at the exponential growth phase, the author added exogenous tyrosol into the cultures at this time so as to produce salidroside through biotransformation. The effects of tyrosol concentration, the way of tyrosol addition as well as the cell density on the transformation rate and salidroside yield were investigated. It was found that the transformation rate attained 95% after cells were incubated in the medium containing 1 mmol/L tyrosol for 24 h. Excess high concentrations of tyrosol in medium (> 3 mmol/L) caused inhibition of transformation rate and cell growth. By 3 repeated additions of tyrosol in low concentrations, the salidroside yields of 1 320 mg/L, 1 740 mg/L and 1 980 mg/L to the cell densities of 6 g DW/L, 12 g DW/L and 18 g DW/L were obtained respectively. |
| Keyword | Rhodiola Sachalinensis Salidroside Tyrosol Biotransformation |
| Subtype | Article |
| WOS Headings | Science & Technology ; Life Sciences & Biomedicine |
| URL | 查看原文 |
| Indexed By | SCI |
| Language | 英语 |
| WOS Keyword | HYDROQUINONE |
| WOS Research Area | Biochemistry & Molecular Biology ; Plant Sciences |
| WOS Subject | Biochemistry & Molecular Biology ; Plant Sciences |
| WOS ID | WOS:000077833800008 |
| Citation statistics | |
| Document Type | 期刊论文 |
| Version | 出版稿 |
| Identifier | http://ir.ipe.ac.cn/handle/122111/6037 |
| Collection | 研究所（批量导入） |
| Affiliation | 1.Chinese Acad Sci, Inst Chem Met, State Key Lab Biochem Engn, Beijing 100080, Peoples R China 2.Dalian Univ Technol, Inst Biochem Engn, Dalian 116012, Peoples R China |
| Recommended Citation GB/T 7714 | Xu, JF,Su, ZG,Feng, PS. Production of salidroside through biotransformation of exogenous tyrosol in Rhodiola sachalinensis cell suspension cultures[J]. ACTA BOTANICA SINICA,1998,40(12):1129-1135. |
| APA | Xu, JF,Su, ZG,&Feng, PS.(1998).Production of salidroside through biotransformation of exogenous tyrosol in Rhodiola sachalinensis cell suspension cultures.ACTA BOTANICA SINICA,40(12),1129-1135. |
| MLA | Xu, JF,et al."Production of salidroside through biotransformation of exogenous tyrosol in Rhodiola sachalinensis cell suspension cultures".ACTA BOTANICA SINICA 40.12(1998):1129-1135. |
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| 利用高山红景天培养细胞生物转化外源酪醇生（225KB） | 限制开放 | CC BY-NC-SA | Application Full Text | |||
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