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Production of salidroside through biotransformation of exogenous tyrosol in Rhodiola sachalinensis cell suspension cultures
Alternative TitleActa Bot. Sin.
Xu, JF; Su, ZG; Feng, PS
1998
Source PublicationACTA BOTANICA SINICA
ISSN0577-7496
Volume40Issue:12Pages:1129-1135
AbstractIn Rhodiola sachalinensis A. Bor. cell cultures, low yields of salidroside was supposed to be associated with the low efficiency of glucosylation reaction at the stationary phase of cell growth, when large amounts of the substrate, aglycon tyrosol, were accumulated. Considering the activity of tyrosol glucosyltransferase being the highest at the exponential growth phase, the author added exogenous tyrosol into the cultures at this time so as to produce salidroside through biotransformation. The effects of tyrosol concentration, the way of tyrosol addition as well as the cell density on the transformation rate and salidroside yield were investigated. It was found that the transformation rate attained 95% after cells were incubated in the medium containing 1 mmol/L tyrosol for 24 h. Excess high concentrations of tyrosol in medium (> 3 mmol/L) caused inhibition of transformation rate and cell growth. By 3 repeated additions of tyrosol in low concentrations, the salidroside yields of 1 320 mg/L, 1 740 mg/L and 1 980 mg/L to the cell densities of 6 g DW/L, 12 g DW/L and 18 g DW/L were obtained respectively.; In Rhodiola sachalinensis A. Bor. cell cultures, low yields of salidroside was supposed to be associated with the low efficiency of glucosylation reaction at the stationary phase of cell growth, when large amounts of the substrate, aglycon tyrosol, were accumulated. Considering the activity of tyrosol glucosyltransferase being the highest at the exponential growth phase, the author added exogenous tyrosol into the cultures at this time so as to produce salidroside through biotransformation. The effects of tyrosol concentration, the way of tyrosol addition as well as the cell density on the transformation rate and salidroside yield were investigated. It was found that the transformation rate attained 95% after cells were incubated in the medium containing 1 mmol/L tyrosol for 24 h. Excess high concentrations of tyrosol in medium (> 3 mmol/L) caused inhibition of transformation rate and cell growth. By 3 repeated additions of tyrosol in low concentrations, the salidroside yields of 1 320 mg/L, 1 740 mg/L and 1 980 mg/L to the cell densities of 6 g DW/L, 12 g DW/L and 18 g DW/L were obtained respectively.
KeywordRhodiola Sachalinensis Salidroside Tyrosol Biotransformation
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
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Indexed BySCI
Language英语
WOS KeywordHYDROQUINONE
WOS Research AreaBiochemistry & Molecular Biology ; Plant Sciences
WOS SubjectBiochemistry & Molecular Biology ; Plant Sciences
WOS IDWOS:000077833800008
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Cited Times:1[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Version出版稿
Identifierhttp://ir.ipe.ac.cn/handle/122111/6037
Collection研究所(批量导入)
Affiliation1.Chinese Acad Sci, Inst Chem Met, State Key Lab Biochem Engn, Beijing 100080, Peoples R China
2.Dalian Univ Technol, Inst Biochem Engn, Dalian 116012, Peoples R China
Recommended Citation
GB/T 7714
Xu, JF,Su, ZG,Feng, PS. Production of salidroside through biotransformation of exogenous tyrosol in Rhodiola sachalinensis cell suspension cultures[J]. ACTA BOTANICA SINICA,1998,40(12):1129-1135.
APA Xu, JF,Su, ZG,&Feng, PS.(1998).Production of salidroside through biotransformation of exogenous tyrosol in Rhodiola sachalinensis cell suspension cultures.ACTA BOTANICA SINICA,40(12),1129-1135.
MLA Xu, JF,et al."Production of salidroside through biotransformation of exogenous tyrosol in Rhodiola sachalinensis cell suspension cultures".ACTA BOTANICA SINICA 40.12(1998):1129-1135.
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