CAS OpenIR  > 研究所(批量导入)
PURIFICATION OF INDUSTRIAL ALPHA-AMYLASE BY REVERSED MICELLAR EXTRACTION
Alternative TitleBiotechnol. Bioeng.
CHANG, QL; CHEN, JY
1995-12-20
Source PublicationBIOTECHNOLOGY AND BIOENGINEERING
ISSN0006-3592
Volume48Issue:6Pages:745-748
AbstractThe purification of industrial alpha-amylase by liquid-liquid extraction with Aliquat 336 reversed micellar solution as the extractant was studied. Seven kinds of Aliquat 336 reversed micellar solution, formed by using seven kinds of straight chain alkyl alcohols as cosolvent, have been utilized to extract industrial alpha-amylase. It was found that these seven kinds of reversed micellar solution can all achieve a high protein transfer efficiency in the forward extraction process. After a full forward and backward extraction cycle, however, only the reversed micelles with n-butanol as the cosolvent was found to be able to maintain the activity of alpha-amylase in the stripping solution. By using the reversed micelles of Aliquat 336/isooctane/1% (v/v) n-butanol to perform a full extraction cycle, it was found that 85% of the total activity of alpha-amylase in the industrial alpha-amylase could be recovered at the end of an extraction cycle and the specific activity of alpha-amylase could be concentrated about 1.5-fold; meanwhile, most of the neutral protease in the industrial alpha-amylase could be removed. The separation factor of alpha-amylase to neutral protease at the end of an extraction cycle can reach about 10. (C) 1995 John Wiley & Sons, Inc.; The purification of industrial alpha-amylase by liquid-liquid extraction with Aliquat 336 reversed micellar solution as the extractant was studied. Seven kinds of Aliquat 336 reversed micellar solution, formed by using seven kinds of straight chain alkyl alcohols as cosolvent, have been utilized to extract industrial alpha-amylase. It was found that these seven kinds of reversed micellar solution can all achieve a high protein transfer efficiency in the forward extraction process. After a full forward and backward extraction cycle, however, only the reversed micelles with n-butanol as the cosolvent was found to be able to maintain the activity of alpha-amylase in the stripping solution. By using the reversed micelles of Aliquat 336/isooctane/1% (v/v) n-butanol to perform a full extraction cycle, it was found that 85% of the total activity of alpha-amylase in the industrial alpha-amylase could be recovered at the end of an extraction cycle and the specific activity of alpha-amylase could be concentrated about 1.5-fold; meanwhile, most of the neutral protease in the industrial alpha-amylase could be removed. The separation factor of alpha-amylase to neutral protease at the end of an extraction cycle can reach about 10. (C) 1995 John Wiley & Sons, Inc.
KeywordAliquat 336 Reversed Micelles Purification Alpha-amylase
SubtypeNote
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
URL查看原文
Indexed BySCI
Language英语
WOS KeywordLIQUID-LIQUID-EXTRACTION ; SOLUBILIZATION ; RECOVERY
WOS Research AreaBiotechnology & Applied Microbiology
WOS SubjectBiotechnology & Applied Microbiology
WOS IDWOS:A1995TF74900023
Citation statistics
Cited Times:12[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Version出版稿
Identifierhttp://ir.ipe.ac.cn/handle/122111/6041
Collection研究所(批量导入)
Recommended Citation
GB/T 7714
CHANG, QL,CHEN, JY. PURIFICATION OF INDUSTRIAL ALPHA-AMYLASE BY REVERSED MICELLAR EXTRACTION[J]. BIOTECHNOLOGY AND BIOENGINEERING,1995,48(6):745-748.
APA CHANG, QL,&CHEN, JY.(1995).PURIFICATION OF INDUSTRIAL ALPHA-AMYLASE BY REVERSED MICELLAR EXTRACTION.BIOTECHNOLOGY AND BIOENGINEERING,48(6),745-748.
MLA CHANG, QL,et al."PURIFICATION OF INDUSTRIAL ALPHA-AMYLASE BY REVERSED MICELLAR EXTRACTION".BIOTECHNOLOGY AND BIOENGINEERING 48.6(1995):745-748.
Files in This Item:
File Name/Size DocType Version Access License
PURIFICATION OF INDU(345KB) 限制开放CC BY-NC-SAApplication Full Text
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[CHANG, QL]'s Articles
[CHEN, JY]'s Articles
Baidu academic
Similar articles in Baidu academic
[CHANG, QL]'s Articles
[CHEN, JY]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[CHANG, QL]'s Articles
[CHEN, JY]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.