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A quantitative study on secondary structure of proteins by FT-Raman spectroscopy
Alternative TitleSpectrosc. Spectr. Anal.
Wang, B; Wang, J; Yu, J; Liu, HZ
1999-10-01
Source PublicationSPECTROSCOPY AND SPECTRAL ANALYSIS
ISSN1000-0593
Volume19Issue:5Pages:674-676
AbstractIn this work, proteins have been scanned by FT-Raman spectrometer. Deconvolved spectra second derivative or fourth derivative spectra were employed to determine positions of individual components. Curve-fitting procedures were carried out to amide I band of original spectra. The broad band was resolved into several components. The resolved band components were assigned to specific protein conformations. The areas of individual components are used to estimate the percentage of the relative secondary structure. Results are in reasonable agreement with data obtained by other methods.; In this work, proteins have been scanned by FT-Raman spectrometer. Deconvolved spectra second derivative or fourth derivative spectra were employed to determine positions of individual components. Curve-fitting procedures were carried out to amide I band of original spectra. The broad band was resolved into several components. The resolved band components were assigned to specific protein conformations. The areas of individual components are used to estimate the percentage of the relative secondary structure. Results are in reasonable agreement with data obtained by other methods.
KeywordFt-raman Protein Secondary Structure Curve Fitting
SubtypeArticle
WOS HeadingsScience & Technology ; Technology
URL查看原文
Indexed BySCI
Language英语
WOS Research AreaSpectroscopy
WOS SubjectSpectroscopy
WOS IDWOS:000083532800010
Citation statistics
Cited Times:4[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Version出版稿
Identifierhttp://ir.ipe.ac.cn/handle/122111/6042
Collection研究所(批量导入)
AffiliationChinese Acad Sci, Inst Chem Met, Beijing 100080, Peoples R China
Recommended Citation
GB/T 7714
Wang, B,Wang, J,Yu, J,et al. A quantitative study on secondary structure of proteins by FT-Raman spectroscopy[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS,1999,19(5):674-676.
APA Wang, B,Wang, J,Yu, J,&Liu, HZ.(1999).A quantitative study on secondary structure of proteins by FT-Raman spectroscopy.SPECTROSCOPY AND SPECTRAL ANALYSIS,19(5),674-676.
MLA Wang, B,et al."A quantitative study on secondary structure of proteins by FT-Raman spectroscopy".SPECTROSCOPY AND SPECTRAL ANALYSIS 19.5(1999):674-676.
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FT_Raman光谱对蛋白质二级结构的定(106KB) 限制开放CC BY-NC-SAApplication Full Text
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