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REVERSED MICELLAR EXTRACTION OF TRYPSIN - EFFECT OF SOLVENT ON THE PROTEIN TRANSFER AND ACTIVITY RECOVERY
Alternative TitleBiotechnol. Bioeng.
CHANG, QI; CHEN, JY
1995-04-20
Source PublicationBIOTECHNOLOGY AND BIOENGINEERING
ISSN0006-3592
Volume46Issue:2Pages:172-174
AbstractBy using trypsin as the model protein and AOT as the model surfactant, the effect of a variety of solvents on protein transfer and activity recovery during the liquid-liquid reversed micellar extraction was investigated. It was found that several solvents, including isotane, heptane, and kerosene, had a similar effect on the recovery of trypsin activity after a full cycle of forward and backward extraction, and could all be used as the solvents for AOT-reversed micelles in trypsin extraction. Two other solvents (hexane and cyclohexane), however, were not so efficient. (C) 1995 John Wiley and Sons, Inc.; By using trypsin as the model protein and AOT as the model surfactant, the effect of a variety of solvents on protein transfer and activity recovery during the liquid-liquid reversed micellar extraction was investigated. It was found that several solvents, including isotane, heptane, and kerosene, had a similar effect on the recovery of trypsin activity after a full cycle of forward and backward extraction, and could all be used as the solvents for AOT-reversed micelles in trypsin extraction. Two other solvents (hexane and cyclohexane), however, were not so efficient. (C) 1995 John Wiley and Sons, Inc.
KeywordReversed Micelles Extraction Trypsin
SubtypeNote
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
URL查看原文
Indexed BySCI
Language英语
WOS KeywordLIQUID-LIQUID-EXTRACTION
WOS Research AreaBiotechnology & Applied Microbiology
WOS SubjectBiotechnology & Applied Microbiology
WOS IDWOS:A1995QM75700009
Citation statistics
Cited Times:10[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Version出版稿
Identifierhttp://ir.ipe.ac.cn/handle/122111/6053
Collection研究所(批量导入)
Recommended Citation
GB/T 7714
CHANG, QI,CHEN, JY. REVERSED MICELLAR EXTRACTION OF TRYPSIN - EFFECT OF SOLVENT ON THE PROTEIN TRANSFER AND ACTIVITY RECOVERY[J]. BIOTECHNOLOGY AND BIOENGINEERING,1995,46(2):172-174.
APA CHANG, QI,&CHEN, JY.(1995).REVERSED MICELLAR EXTRACTION OF TRYPSIN - EFFECT OF SOLVENT ON THE PROTEIN TRANSFER AND ACTIVITY RECOVERY.BIOTECHNOLOGY AND BIOENGINEERING,46(2),172-174.
MLA CHANG, QI,et al."REVERSED MICELLAR EXTRACTION OF TRYPSIN - EFFECT OF SOLVENT ON THE PROTEIN TRANSFER AND ACTIVITY RECOVERY".BIOTECHNOLOGY AND BIOENGINEERING 46.2(1995):172-174.
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