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SEPARATION AND PURIFICATION OF 2 ENZYMES FROM BACILLUS-SUBTILIS USING ALIQUAT-336 REVERSED MICELLES - STUDY OF THE EFFECT OF COSOLVENT CONCENTRATION
Alternative TitleChem. Eng. J. Biochem. Eng. J.
CHANG, QL; CHEN, JY
1995-11-01
Source PublicationCHEMICAL ENGINEERING JOURNAL AND THE BIOCHEMICAL ENGINEERING JOURNAL
ISSN0923-0467
Volume59Issue:3Pages:303-308
AbstractTwo kinds of alkyl alcohol (n-butanol and n-octanol) at concentrations ranging from 0.4% to 2.0% (v/v) were chosen as the cosolvent for Aliquat 336/isooctane reversed micelles. By performing liquid-liquid extraction with Aliquat 336 reversed micelles, separation and purification of two enzymes (alpha-amylase and neutral protease) from Bacillus subtilis were investigated. It was found that at higher cosolvent concentration (above 0.4% v/v), n-butanol may be a suitable cosolvent for Aliquat 336 reversed micelles. Under this condition, about 90% of the total activity of alpha-amylase in the crude enzyme preparation can be recovered in the stripping solution after one extraction cycle and the enzyme can be purified about 1.4-fold; meanwhile about 70% of the total activity of neutral protease in the crude enzyme preparation is retrieved into the raffinate with about 4.5-fold purification. At the lower cosolvent concentration range (below 0.4% v/v), however, n-octanol is the better choice. Under this condition, about 85% of the total activity of alpha-amylase can be recovered into the stripping solution and purified by 1.3-fold; meanwhile about 75% of the total activity of neutral protease is transferred into the raffinate with the enzyme purified by 4.0-fold.; Two kinds of alkyl alcohol (n-butanol and n-octanol) at concentrations ranging from 0.4% to 2.0% (v/v) were chosen as the cosolvent for Aliquat 336/isooctane reversed micelles. By performing liquid-liquid extraction with Aliquat 336 reversed micelles, separation and purification of two enzymes (alpha-amylase and neutral protease) from Bacillus subtilis were investigated. It was found that at higher cosolvent concentration (above 0.4% v/v), n-butanol may be a suitable cosolvent for Aliquat 336 reversed micelles. Under this condition, about 90% of the total activity of alpha-amylase in the crude enzyme preparation can be recovered in the stripping solution after one extraction cycle and the enzyme can be purified about 1.4-fold; meanwhile about 70% of the total activity of neutral protease in the crude enzyme preparation is retrieved into the raffinate with about 4.5-fold purification. At the lower cosolvent concentration range (below 0.4% v/v), however, n-octanol is the better choice. Under this condition, about 85% of the total activity of alpha-amylase can be recovered into the stripping solution and purified by 1.3-fold; meanwhile about 75% of the total activity of neutral protease is transferred into the raffinate with the enzyme purified by 4.0-fold.
KeywordReversed Micelles Cosolvent Separation Alpha-amylase Neutral Protease
SubtypeArticle
WOS HeadingsScience & Technology ; Technology
URL查看原文
Indexed BySCI
Language英语
WOS KeywordLIQUID-LIQUID-EXTRACTION ; ALPHA-CHYMOTRYPSIN ; PROTEIN EXTRACTION ; SOLUBILIZATION
WOS Research AreaEngineering
WOS SubjectEngineering, Chemical
WOS IDWOS:A1995TH24000014
Citation statistics
Cited Times:11[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Version出版稿
Identifierhttp://ir.ipe.ac.cn/handle/122111/6059
Collection研究所(批量导入)
Recommended Citation
GB/T 7714
CHANG, QL,CHEN, JY. SEPARATION AND PURIFICATION OF 2 ENZYMES FROM BACILLUS-SUBTILIS USING ALIQUAT-336 REVERSED MICELLES - STUDY OF THE EFFECT OF COSOLVENT CONCENTRATION[J]. CHEMICAL ENGINEERING JOURNAL AND THE BIOCHEMICAL ENGINEERING JOURNAL,1995,59(3):303-308.
APA CHANG, QL,&CHEN, JY.(1995).SEPARATION AND PURIFICATION OF 2 ENZYMES FROM BACILLUS-SUBTILIS USING ALIQUAT-336 REVERSED MICELLES - STUDY OF THE EFFECT OF COSOLVENT CONCENTRATION.CHEMICAL ENGINEERING JOURNAL AND THE BIOCHEMICAL ENGINEERING JOURNAL,59(3),303-308.
MLA CHANG, QL,et al."SEPARATION AND PURIFICATION OF 2 ENZYMES FROM BACILLUS-SUBTILIS USING ALIQUAT-336 REVERSED MICELLES - STUDY OF THE EFFECT OF COSOLVENT CONCENTRATION".CHEMICAL ENGINEERING JOURNAL AND THE BIOCHEMICAL ENGINEERING JOURNAL 59.3(1995):303-308.
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